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Direct Black 38

Name: Direct Black 38
Brand: TargetMol
SKU: T15135
Rating: 4.5 (1 reviews)

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Catalog No. T15135Cas No. 1937-37-7

Alias Chlorazol Black E, C.I. 30235

Direct Black 38 (Chlorazol Black E) is a black azo dye that absorbs various colors of light and is commonly used in the textile industry.Direct Black 38 is genotoxic and induces unplanned DNA synthesis in rat liver and bone marrow micronuclei.

Direct Black 38

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Catalog No. T15135Alias Chlorazol Black E, C.I. 30235Cas No. 1937-37-7

Pack Size	Price	USA Warehouse	Global Warehouse
10 g	$105	35 days	35 days
25 g	$163	35 days	35 days
50 g	$297	35 days	35 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Appearance:Solid

Color:Black

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COA

Product Introduction

Bioactivity

Description	Direct Black 38 (Chlorazol Black E) is a black azo dye that absorbs various colors of light and is commonly used in the textile industry.Direct Black 38 is genotoxic and induces unplanned DNA synthesis in rat liver and bone marrow micronuclei.
Cell Research	Instructions I. Solution preparation 1. Recommended concentration: 1–5% (mass/volume). Dissolve in deionized water and dilute as needed. Storage conditions: The stock solution is stable under refrigerated conditions at 4°C. Avoid prolonged exposure to strong light or high temperatures to prevent degradation. II. Operation steps 1. Textile dyeing 1). Prepare the dye bath: Heat the dye solution in the range of 60–90°C and maintain uniform mixing. Adjust the dye concentration according to the material and the expected color depth. 2) Immerse the material in the dye bath, usually for 30–60 minutes. 3) Fix and wash: Use an appropriate amount of salt or alkaline substances (such as sodium carbonate) as an auxiliary agent to enhance binding. 4) Wash after dyeing to remove excess dye. 2. Biological experiments 1) Cell or tissue staining: Dilute the stock solution (working concentration is 0.1–1%). 2) Directly apply or immerse in the sample, the staining time can be optimized to 5–30 minutes. 3) After staining, rinse with appropriate buffer (such as PBS) to remove unbound dye. 4) Microscope observation: Usually observed under bright field microscope, choose appropriate light source and filter to improve contrast. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Chlorazol Black E, C.I. 30235

Chemical Properties

Molecular Weight	781.73
Formula	C₃₄H₂₅N₉Na₂O₇S₂
Cas No.	1937-37-7
Smiles	Nc1ccc(N=Nc2ccc(-c3ccc(N=Nc4c(S(=O)(=O)[O-])cc5cc(S(=O)(=O)[O-])c(N=Nc6ccccc6)c(O)c5c4N)cc3)cc2)c(N)c1.[Na+].[Na+]
Relative Density.	1.61g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 20 mg/mL (25.58 mM), Sonication is recommended.

H2O: 2 mg/mL (2.56 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (2.56 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.2792 mL	6.3961 mL	12.7921 mL	63.9607 mL

DMSO

	1mg	5mg	10mg	50mg
5 mM	0.2558 mL	1.2792 mL	2.5584 mL	12.7921 mL
10 mM	0.1279 mL	0.6396 mL	1.2792 mL	6.3961 mL
20 mM	0.0640 mL	0.3198 mL	0.6396 mL	3.1980 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc