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DDO-2728

Name: DDO-2728
Brand: TargetMol
SKU: T205009
Rating: 4.5 (1 reviews)

(Synonyms: DDO2728, DDO 2728) Copy Product Info

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Synonyms: DDO2728, DDO 2728

Catalog No. T205009 Copy Product Info

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DDO-2728 is a selective pyrazolo[1,5-a]pyrimidine-based ALKBH5 inhibitor with an IC50 of 2.97 μM. It increases the levels of N6-methyladenosine (m6A) modifications in acute myeloid leukemia (AML) cells and exhibits antiproliferative activity in these cells. DDO-2728 also demonstrates anti-tumor efficacy in the MV4-11 xenograft model.

Cas No. 3029515-97-4

Pack Size	Price	USA Stock	Global Stock	Quantity
10 mg	Inquiry	10-14 weeks	10-14 weeks
50 mg	Inquiry	10-14 weeks	10-14 weeks

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	DDO-2728 is a selective pyrazolo[1,5-a]pyrimidine-based ALKBH5 inhibitor with an IC50 of 2.97 μM. It increases the levels of N6-methyladenosine (m6A) modifications in acute myeloid leukemia (AML) cells and exhibits antiproliferative activity in these cells. DDO-2728 also demonstrates anti-tumor efficacy in the MV4-11 xenograft model.
Targets & IC50	HUVEC:165.1 μM, HEK293 cells:97.4 μM, MOLM-13 cells:0.45 μM, MV4-11 cells:1.2 μM
In vitro	DDO-2728 (0–40 μM, 48 h) increased m6A methylation levels in MOLM-13, HEK293, and MV4-11 cells across a concentration gradient [1]. DDO-2728 (0.01–100 μM, 72 h) inhibits the proliferation of MOLM-13 and MV4-11 cells, with IC₅₀ values of 0.45 and 1.2 μM, respectively, and exhibits relatively low toxicity toward HEK293 and HUVEC cells [1]. DDO-2728 (20 μM, 48 h) significantly arrested the cell cycle of MOLM-13 and MV4-11 cells at the G1/M phase [1]. DDO-2728 (5, 10 μM, 48 h) induced apoptosis in MV4-11 and MOLM-13 cells in a concentration-dependent manner [1]. DDO-2728 (20 μM, 24 h) reduced the half-life of TACC3 mRNA in MOLM-13 and MV4-11 cells [1]. DDO-2728 (0–10 μM, 48 h) significantly reduced the abundance of TACC3 and c-Myc in MOLM-13 and MV4-11 cells at both the mRNA and protein levels [1].
In vivo	DDO-2728 (10–40 mg/kg, intraperitoneal injection, once daily, for 14 days) effectively inhibited tumor growth in nude mice with MV4-11 xenografts [1].
Synonyms	DDO2728, DDO 2728

Chemical Properties

Molecular Weight	578.45
Formula	C₂₈H₁₇F₃N₄O₇
Cas No.	3029515-97-4
Smiles	O=C(C1=CC=C(C2=NN3C=C(C(C4=C(O)C([N+]([O-])=O)=CC=C4O)=O)C=NC3=C2)C=C1)OCC5=CC=C(C(F)(F)F)C=C5

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100.00 mg/mL (172.88 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7288 mL	8.6438 mL	17.2876 mL	86.4379 mL
5 mM	0.3458 mL	1.7288 mL	3.4575 mL	17.2876 mL
10 mM	0.1729 mL	0.8644 mL	1.7288 mL	8.6438 mL
20 mM	0.0864 mL	0.4322 mL	0.8644 mL	4.3219 mL
50 mM	0.0346 mL	0.1729 mL	0.3458 mL	1.7288 mL
100 mM	0.0173 mL	0.0864 mL	0.1729 mL	0.8644 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Related Tags: DDO-2728 chemical structure | DDO-2728 in vivo | DDO-2728 in vitro | DDO-2728 formula | DDO-2728 molecular weight