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DC-5163

Name: DC-5163
Brand: TargetMol
SKU: T10966
Rating: 4.5 (1 reviews)

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Catalog No. T10966Cas No. 897771-47-0

DC-5163 is a potent inhibitor of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with IC50 of 176.3 nM and Kd of 3.192 μM. DC-5163 selectively inhibits cancer cell proliferation and induces apoptosis, and partially inhibits the glycolysis pathway.

DC-5163

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Catalog No. T10966Cas No. 897771-47-0

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,520	6-8 weeks	6-8 weeks
50 mg	$1,980	6-8 weeks	6-8 weeks
100 mg	$2,500	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	DC-5163 is a potent inhibitor of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with IC50 of 176.3 nM and Kd of 3.192 μM. DC-5163 selectively inhibits cancer cell proliferation and induces apoptosis, and partially inhibits the glycolysis pathway.
Targets&IC50	GAPDH:176.3 nM , GAPDH:(kd)3.192 μM
In vitro	DC-5163 (100 μM; 48 hours; MDA-MB-231 cells) treatment can obviously induce apoptosis of MDA-MB-231 cells. DC-5163 inhibited the proliferation of MDA-MB-231 cells with an IC50 of 49.22 μM at 48 hours and 52.09 μM at 72 hours. Compared with the experimental control, DC-5163 also significantly reduced glucose uptake, lactic acid production and 18F-FDG uptake rate. After treatment with 25μM DC-5163 for 48 hours, it can inhibit the GAPDH activity of BT-549 cells, MCF7 cells, HCT116 cells, MDA-MB-231 cells and A549 cells.

Chemical Properties

Molecular Weight	361.89
Formula	C₁₈H₂₀ClN₃OS
Cas No.	897771-47-0
Smiles	C(N1CN(C(NC2=CC(Cl)=C(OC)C=C2)=S)CC1)C3=CC=CC=C3
Relative Density.	1.332 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 300 mg/mL (828.98 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 5 mg/mL (13.82 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7633 mL	13.8164 mL	27.6327 mL	138.1635 mL
5 mM	0.5527 mL	2.7633 mL	5.5265 mL	27.6327 mL
10 mM	0.2763 mL	1.3816 mL	2.7633 mL	13.8164 mL
20 mM	0.1382 mL	0.6908 mL	1.3816 mL	6.9082 mL
50 mM	0.0553 mL	0.2763 mL	0.5527 mL	2.7633 mL
100 mM	0.0276 mL	0.1382 mL	0.2763 mL	1.3816 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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DC-5163

DC-5163

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In Vivo Formulation Calculator (Clear solution)

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