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Bathophenanthroline

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Catalog No. T18919Cas No. 1662-01-7

Bathophenanthroline is a compound used for the measurement of iron concentrations in aqueous, urine, and serum samples by colorimetry.

Bathophenanthroline

Bathophenanthroline

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Purity: 99.71%
Catalog No. T18919Cas No. 1662-01-7
Bathophenanthroline is a compound used for the measurement of iron concentrations in aqueous, urine, and serum samples by colorimetry.
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500 mg$29-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:99.71%
Appearance:Solid
Color:White
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Product Introduction

Bathophenanthroline AI Summary
Bathophenanthroline demonstrates notable bioactivity by inhibiting the proliferation of both drug-resistant Plasmodium falciparum strain W2 and drug-susceptible strain 3D7 in erythrocyte-based infection assays, with EC50 values of 430.0 nM and 329.0 nM, respectively. It also exhibits low cytotoxicity against the Huh7 human hepatocellular carcinoma cell line, with a CC50 greater than 100,000.0 nM. In high-throughput screening assays, Bathophenanthroline shows significant inhibitory or inductive activity (>50%) with an Inhibition Frequency Index (IFI) of 13.74%. Additionally, the compound effectively inhibits growth in various human tumor cell lines, with varying GI50 values suggesting its potential efficacy against cancer. Specifically, it inhibits light emission in HepG2 cells infected with luciferase-expressing antifolate-resistant parasites, with an IC50 of 1.39 µM, while also displaying hepatotoxicity in the HEPG2TOX assay at a CC50 of 1.39 µM. These properties indicate that Bathophenanthroline has promise as both an antiparasitic and anticancer agent, though its hepatotoxic effects warrant further investigation..
Note: Summary generated by AI. Data source: ChEMBL
Bioactivity
Description
Bathophenanthroline is a compound used for the measurement of iron concentrations in aqueous, urine, and serum samples by colorimetry.
In vitro
Iron concentration determination
Operation steps:
1. Prepare the standard iron solution and the required Bathophenanthroline reagent solution (10-250uM).
2. Add Bathophenanthroline reagent to the sample to be tested.
3. Perform spectrophotometric measurements of the mixture within a spectral range of appropriate wavelengths (typically 535 nm).
4. Calculate the iron concentration in the sample according to the standard curve.
5. Analyze the iron concentration in water, serum or urine samples.
Treatment of water and serum samples: Bathophenanthroline can react directly with the sample when the iron content is low; however, when the iron content is high, the sample may need to be diluted and the concentration of the reagent may be adjusted according to the experimental requirements.
Notes:
1. Make sure there are no other interfering substances in the experiment, especially other metal ions that may react with Bathophenanthroline.
2. The reaction of Bathophenanthroline reagent is sensitive to environmental conditions (such as temperature, pH) and should be operated under appropriate conditions.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Chemical Properties
Molecular Weight332.4
FormulaC24H16N2
Cas No.1662-01-7
SmilesC1=CC=C(C=C1)C1=CC=NC2=C1C=CC1=C2N=CC=C1C1=CC=CC=C1
Relative Density.1.21g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: < 0.1 mg/mL (insoluble)
DMSO: 2.5 mg/mL (7.52 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM3.0084 mL15.0421 mL30.0842 mL150.4212 mL
5 mM0.6017 mL3.0084 mL6.0168 mL30.0842 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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