Bathophenanthroline is a compound used for the measurement of iron concentrations in aqueous, urine, and serum samples by colorimetry.

Iron concentration determination
Operation steps:
1. Prepare the standard iron solution and the required Bathophenanthroline reagent solution (10-250uM).
2. Add Bathophenanthroline reagent to the sample to be tested.
3. Perform spectrophotometric measurements of the mixture within a spectral range of appropriate wavelengths (typically 535 nm).
4. Calculate the iron concentration in the sample according to the standard curve.
5. Analyze the iron concentration in water, serum or urine samples.
Treatment of water and serum samples: Bathophenanthroline can react directly with the sample when the iron content is low; however, when the iron content is high, the sample may need to be diluted and the concentration of the reagent may be adjusted according to the experimental requirements.
Notes:
1. Make sure there are no other interfering substances in the experiment, especially other metal ions that may react with Bathophenanthroline.
2. The reaction of Bathophenanthroline reagent is sensitive to environmental conditions (such as temperature, pH) and should be operated under appropriate conditions.