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Bathophenanthroline is a compound used for the measurement of iron concentrations in aqueous, urine, and serum samples by colorimetry.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 500 mg | $29 | - | In Stock |

| Description | Bathophenanthroline is a compound used for the measurement of iron concentrations in aqueous, urine, and serum samples by colorimetry. |
| In vitro | Iron concentration determination Operation steps: 1. Prepare the standard iron solution and the required Bathophenanthroline reagent solution (10-250uM). 2. Add Bathophenanthroline reagent to the sample to be tested. 3. Perform spectrophotometric measurements of the mixture within a spectral range of appropriate wavelengths (typically 535 nm). 4. Calculate the iron concentration in the sample according to the standard curve. 5. Analyze the iron concentration in water, serum or urine samples. Treatment of water and serum samples: Bathophenanthroline can react directly with the sample when the iron content is low; however, when the iron content is high, the sample may need to be diluted and the concentration of the reagent may be adjusted according to the experimental requirements. Notes: 1. Make sure there are no other interfering substances in the experiment, especially other metal ions that may react with Bathophenanthroline. 2. The reaction of Bathophenanthroline reagent is sensitive to environmental conditions (such as temperature, pH) and should be operated under appropriate conditions. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 332.4 |
| Formula | C24H16N2 |
| Cas No. | 1662-01-7 |
| Smiles | C1=CC=C(C=C1)C1=CC=NC2=C1C=CC1=C2N=CC=C1C1=CC=CC=C1 |
| Relative Density. | 1.21g/cm3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||
| Solubility Information | H2O: < 0.1 mg/mL (insoluble) DMSO: 2.5 mg/mL (7.52 mM), Sonication is recommended. | |||||||||||||||
Solution Preparation Table | ||||||||||||||||
DMSO
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Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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