Apoptosis inducer41 is an apoptosis-inducing agent that prompts cell death through the mitochondrial pathway. It exhibits significant inhibitory effects on MCF-7 cells, with an IC50 of 6.2 μM. Apoptosis inducer41 notably arrests MCF-7 cells in the G2/M phase, increases the accumulation of reactive oxygen species (ROS), and induces mitochondrial membrane potential depolarization. This compound is applicable in breast cancer research.

Apoptosis inducer 41 (Compound 6j) exhibits antiproliferative activity across various cell lines, with IC50 values of 7.1, 40.9, 6.2, and 46.1 μM for MDA-MB-231, MDA-MB-468, MCF-7, and MCF-10A cells, respectively, over a 48-hour period at concentrations of 1-100 μM. In MCF-7 cells, it effectively induces apoptosis through the mitochondrial pathway, causing cell cycle arrest at the G2/M phase at 1-9 μM concentrations over 24-48 hours. Additionally, Apoptosis inducer 41 (1-9 μM, 24 h) generates reactive oxygen species (ROS) and disrupts mitochondrial membrane potential (MMP).