Antitumor agent-204 (compound 15a) is a potent antitumor drug with cytotoxic properties. It induces the release of nitric oxide (NO) and the production of reactive oxygen species (ROS). Additionally, Antitumor agent-204 triggers apoptosis and causes cell cycle arrest at the G2/M phase, demonstrating significant antitumor activity.

Antitumor agent-204 (compound 15a) exhibits cytotoxicity against A549, A549/Taxol, MDA-MB-231, MCF-7, BEAS-2B, and HUVEC cells with IC50 values of 4.43, 0.87, 1.20, 5.54, 10.73, and 13.25 μM, respectively, when applied at concentrations ranging from 0 to 10 μM for 48 hours. Additionally, at concentrations of 1, 2.5, and 5 μM over 24 hours, it induces cell apoptosis in a dose-dependent manner.

Antitumor agent-204, administered intraperitoneally at doses of 1, 5, and 10 mg/kg every four days over a 16-day period, has demonstrated antitumor activity in mice.