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AMPC

Catalog No. T39792 CAS 2254434-33-6

AMPC, a powerful and efficient inhibitor of TFF3, demonstrates inhibitory effects on cell proliferation, survival, oncogenicity, and CSC-like behavior in TFF3-positive CMS4 CRC cells. It acts as a promising standalone anti-cancer agent and also enhances the effects of 5-FU when used in combination, making it a valuable tool for cancer research.

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AMPC, CAS 2254434-33-6

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Biological Description

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Storage & Solubility Information

Description	AMPC, a powerful and efficient inhibitor of TFF3, demonstrates inhibitory effects on cell proliferation, survival, oncogenicity, and CSC-like behavior in TFF3-positive CMS4 CRC cells. It acts as a promising standalone anti-cancer agent and also enhances the effects of 5-FU when used in combination, making it a valuable tool for cancer research.
In vitro	AMPC effectively inhibits cell proliferation in CMS4 CRC cells, with IC50 values of 2.63 μM, 4.65 μM, and 69.69 μM for SW620 (high TFF3 expression), Caco-2, and SW480 (low TFF3 expression) cells, respectively[1]. Additionally, AMPC, at concentrations ranging from 1-10 μM, significantly lowers TFF3 levels in SW620 and Caco2 cells in a dose-dependent manner[1]. Moreover, AMPC promotes apoptosis through TFF3 inhibition, characterized by a reduction in the S-phase cell population and an increase in caspase3/7 activity, compared to control groups[1].
In vivo	AMPC administered via intraperitoneal injection at a dosage of 40 mg/kg daily significantly diminishes tumor volume in AMPC-treated mice compared to those receiving the vehicle, observable from day 11 onwards. It promotes considerable tumor necrosis and an increase in apoptotic cell features within SW620 tumors. Furthermore, AMPC notably lowers both tumor and serum TFF3 levels in vivo. In this study, SW620 cells were subcutaneously inoculated into nude mice, highlighting AMPC's potential in reducing Ki67 expression in tumors, thereby underscoring its efficacy in tumor suppression and induction of cell death pathways.

Molecular Weight	425.419
Formula	C25H16FN3O3
CAS No.	2254434-33-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Ru-Mei Chen, et al. Pharmacological Inhibition of TFF3 Enhances Sensitivity of CMS4 Colorectal Carcinoma to 5-Fluorouracil through Inhibition of p44/42 MAPK. Int J Mol Sci. 2019 Dec 9;20(24):6215

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

AMPC 2254434-33-6 inhibitor inhibit

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