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Sulfo-NHS-SS-Biotin sodium

Catalog No. T19061 CAS 325143-98-4

Synonyms: Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate, Sulfo-NThiol-SS-biotin, Sulfo-NHS-SS-Biotin (sodium), Sulfo-NHS-SS-Biotin

Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.

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Sulfo-NHS-SS-Biotin sodium Chemical Structure

Sulfo-NHS-SS-Biotin sodium, CAS 325143-98-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 32.00
10 mg	In stock	$ 50.00
50 mg	In stock	$ 132.00

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Purity: 99.57%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.
Targets&IC50	Cell-surface protein:
In vitro	Sulfo-NHS-SS-biotin is a negatively charged reagent and does not permeate cell membranes. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1]. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail [2].

Synonyms	Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate, Sulfo-NThiol-SS-biotin, Sulfo-NHS-SS-Biotin (sodium), Sulfo-NHS-SS-Biotin
Molecular Weight	606.69
Formula	C19H27N4NaO9S4
CAS No.	325143-98-4

Storage

store at low temperature,keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 180 mg/mL (296.7 mM), Sonication is recommended.

DMSO: 100 mg/mL (164.8 mM), Sonication is recommended.

References and Literature

1. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26. 2. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Sulfo-NHS-SS-Biotin sodium 325143-98-4 Others Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate Sulfo-NThiol-SS-biotin Sulfo-NHS-SS-Biotin (sodium) Sulfo-NHS-SS-Biotin inhibitor inhibit

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