store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 g | In stock | $ 33.00 | |
5 g | In stock | $ 97.00 |
Description | ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity. |
Targets&IC50 | β-gal:2.7±0.3 mM(Km), β-gal:464.7±7.8/s(kcat) |
In vitro | The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1]. |
Synonyms | 2-Nitrophenyl β-D-galactopyranoside |
Molecular Weight | 301.25 |
Formula | C12H15NO8 |
CAS No. | 369-07-3 |
store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 7.4 mg/mL (24.56 mM), Sonication is recommended.
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ONPG 369-07-3 Others inhibit 2-Nitrophenyl b-D-galactopyranoside Inhibitor 2-Nitrophenyl β-D-galactopyranoside 2-Nitrophenyl beta-D-galactopyranoside inhibitor