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CFSE

Catalog No. T6802   CAS 150347-59-4
Synonyms: 5(6)-CFDA N-succinmidyl ester, Carboxyfluorescein succinimidyl ester, CFDA-SE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester

CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CFSE Chemical Structure
CFSE, CAS 150347-59-4
Pack Size Availability Price/USD Quantity
5 mg In stock $ 52.00
10 mg In stock $ 80.00
25 mg In stock $ 133.00
50 mg In stock $ 198.00
100 mg In stock $ 293.00
200 mg In stock $ 438.00
1 mL * 10 mM (in DMSO) In stock $ 74.00
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Purity: 99.13%
Purity: 98.98%
Purity: 97.94%
Purity: 95.91
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Biological Description
Chemical Properties
Storage & Solubility Information
Description CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.
In vitro METHODS: 1 mL of cells and CFDA (5 mM in 110 μL PBS) were flipped and mixed to label the cells. CFSE-labeled CD8+OT-I T cells were cultured with dendritic cells pulsed with varying amounts of OVA for 3 days, and CFSE profiles were examined using Flow Cytometry.
RESULTS: CD8+ T cells divided 1-3 times according to the CFSE dilution peak, and more T cells divided at higher antigen concentrations. [1]
METHODS: Human erythroleukemia cell line K562, mouse lymphoma cell line YAC-1, human breast cancer cell line MCF-7, and human melanoma cell line A375 were treated with CFDA (1-10 mM) for 1-6 h. Cell death was detected by Flow Cytometry.
RESULTS: CFDA was non-toxic to the cells, as the cell death rate due to CFDA labeling was less than 5%. [2]
In vivo METHODS: CFSE-labeled CD8+OT-I T cells were injected intravenously into the tail of C56BL/6J mice, followed by intravenous injection of OVA (20 μg), and CFSE profiles were measured three days later.
RESULTS: Most of the cells fell within 7 CFSE peaks, indicating that the cells had undergone up to 6 divisions. [1]
METHODS: To label thymocytes in vivo, CFDA (10 μM) was injected into the thymic lobes of anesthetized C56BL/6 mice.
RESULTS: CFDA labeled a representative sample of all thymocyte subpopulations and these cells migrated to peripheral lymphoid organs at a rate of approximately 2-3 x 10^6 cells/day. They enter the lymph nodes on day 1 post-injection and remain there for at least 21 days, while turnover is faster in the spleen. [3]
Cell Research The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 μM, 3 μM, 4 μM, 5 μM, 10 μM and 20 μM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.(Only for Reference)
Synonyms 5(6)-CFDA N-succinmidyl ester, Carboxyfluorescein succinimidyl ester, CFDA-SE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester
Molecular Weight 557.46
Formula C29H19NO11
CAS No. 150347-59-4

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (166.8 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Quah BJ, et al. The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation. J Vis Exp. 2010 Oct 12;(44):2259. 2. Wang XQ, et al. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochim Biophys Sin (Shanghai). 2005 Jun;37(6):379-85. 3. Graziano M, et al. The fate of thymocytes labeled in vivo with CFSE. Exp Cell Res. 1998 Apr 10;240(1):75-85. 4. Li M, Qin M, Song G, et al. A biomimetic antitumor nanovaccine based on biocompatible calcium pyrophosphate and tumor cell membrane antigens[J]. Asian Journal of Pharmaceutical Sciences. 2020

TargetMolCitations

1. Guo E, Xiao R, Wu Y, et al. WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway. Journal of Experimental Medicine. 2021, 219(1): e20210789. 2. Liao Y, Huang J, Liu P, et al. Downregulation of LNMAS orchestrates partial EMT and immune escape from macrophage phagocytosis to promote lymph node metastasis of cervical cancer. Oncogene. 2022: 1-13. 3. Xiao C, Su J, Zhang C, et al. Effectiveness of Booster Doses of the SARS-CoV-2 Inactivated Vaccine KCONVAC against the Mutant Strains. Viruses. 2022, 14(9): 2016. 4. Xiao C, Mao L, Wang Z, et al. SARS-CoV-2 variant B. 1.1. 7 caused HLA-A2+ CD8+ T cell epitope mutations for impaired cellular immune response. Iscience. 2022 Mar 18;25(3):103934. doi: 10.1016/j.isci.2022.103934. Epub 2022 Feb 17. 5. Deng J, Pan J, Qiu M, et al. Identification of HLA-A2 restricted CD8+ T cell epitopes in SARS-CoV-2 structural proteins. Journal of Leukocyte Biology. 2021 6. Jablonski S, Mou H, Otsuka Y, et al. Identification of Potent Small Molecule Inhibitors of SARS-CoV-2 Entry. SLAS Discovery. 2021 7. Zhao G, Tong Y, Xu J, et al.Jing-Fang powder ethyl acetate extracts attenuate atopic dermatitis by modulating T-cell activity.Molecular Immunology.2023, 160: 133-149. 8. Xiao C, Ren Z, Zhang B, et al.Insufficient epitope-specific T cell clones are responsible for impaired cellular immunity to inactivated SARS-CoV-2 vaccine in older adults.Nature Aging.2023: 1-18. 9. Yang L, Wu J, Mei G, et al.Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2.Microbes and Infection.2023: 105244. 10. Li P, Xie Y, Wang J, et al.Gene engineered exosome reverses T cell exhaustion in cancer immunotherapy.Bioactive Materials.2024, 34: 466-481.

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Keywords

CFSE 150347-59-4 Others Inhibitor 5(6)-CFDA N-succinmidyl ester Carboxyfluorescein succinimidyl ester CFDA-SE 5(6)-Carboxyfluorescein diacetate succinimidyl ester inhibit inhibitor

 

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