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A 83-01

A 83-01
A 83-01 (ALK5 Inhibitor IV) is an inhibitor of the TGF-β type I receptors ALK5, ALK4, and ALK7 (IC50=12/45/7.5 nM). A 83-01 promotes the reprogramming of mouse fibroblasts into iPSCs. A 83-01 can be used in organoid cultures.
Catalog No. T3031Cas No. 909910-43-6
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Purity:98.20%
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A 83-01

Catalog No. T3031Cas No. 909910-43-6

A 83-01 (ALK5 Inhibitor IV) is an inhibitor of the TGF-β type I receptors ALK5, ALK4, and ALK7 (IC50=12/45/7.5 nM). A 83-01 promotes the reprogramming of mouse fibroblasts into iPSCs. A 83-01 can be used in organoid cultures.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
5 mg$52In Stock
10 mg$88In Stock
25 mg$165In Stock
50 mg$269In Stock
100 mg$398In Stock
500 mg$939In Stock
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Product Introduction

Bioactivity
Description
A 83-01 (ALK5 Inhibitor IV) is an inhibitor of the TGF-β type I receptors ALK5, ALK4, and ALK7 (IC50=12/45/7.5 nM). A 83-01 promotes the reprogramming of mouse fibroblasts into iPSCs. A 83-01 can be used in organoid cultures.
Targets&IC50
ALK5:12 nM, ALK4:45 nM, ALK7:7.5 nM
In vitro
METHODS: Wild-type mink lung epithelial cells, Mv1Lu, were treated with A 83-01 (0.03-10 μM) and TGF-β (1 ng/mL) for 48 h, and cell proliferation was detected using a Coulter counter.
RESULTS: A 83-01 prevented the inhibition of Mv1Lu cell growth by TGF-β in a dose-dependent manner. [1]
METHODS: Mouse ovarian cancer cells HM-1 were treated with A 83-01 (1-10 μM) for 30 min, followed by treatment with TGF-β (1-10 ng/mL) for 60 min, and the expression levels of target proteins were detected using Western Blot.
RESULTS: The addition of TGF-β1 increased the expression of pSmad3, and A 83-01 inhibited the up-regulation of TGF-β. [2]
In vivo
METHODS: To detect anti-tumor activity in vivo, A 83-01 (150 μg/each) was administered intraperitoneally three times a week for four weeks to a B6C3F1 mouse model of peritoneal spread of HM-1 cancer.
RESULTS: Ascites formation tended to be slower in the A 83-01-treated group, and A 83-01 significantly improved the survival rate of the mice. [2]
METHODS: To investigate the role in myocardial injury, A 83-01 (10 mg/kg) was administered intraperitoneally to Nkx2.5 enh-Cre/mTmG mice once daily for seven days.
RESULTS: A 83-01 treatment significantly increased the number of Nkx2.5+ myocardial myofibroblasts at baseline and after myocardial injury, leading to an increase in the number of newly formed myocardial cells. A 83-01 treatment significantly improved ventricular elasticity and stroke work, which led to an improvement of contractility after injury. [3]
Cell Research
HM-1 cells are seeded into a 96-well plate and are incubated for 18 hr. A-83-01 (1 μM) or vehicle are then added for 12 hr followed by the addition of TGF-β1 (1 ng/mL) or vehicle for 60 hr. The number of viable cells in each well is examined using the WST-1 assay following the manufacturer's instructions.
AliasALK5 Inhibitor IV, A8301
Chemical Properties
Molecular Weight421.52
FormulaC25H19N5S
Cas No.909910-43-6
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | Shipping with blue ice.
Solubility Information
DMSO: 10 mg/mL (23.72 mM), The compound is unstable in solution. Please use soon.
10% DMSO+90% Saline: 1 mg/mL (2.37 mM), Working solution is recommended to be prepared and used immediately.
Solution Preparation Table
10% DMSO+90% Saline/DMSO
1mg5mg10mg50mg
1 mM2.3724 mL11.8618 mL23.7237 mL118.6183 mL
DMSO
1mg5mg10mg50mg
5 mM0.4745 mL2.3724 mL4.7447 mL23.7237 mL
10 mM0.2372 mL1.1862 mL2.3724 mL11.8618 mL
20 mM0.1186 mL0.5931 mL1.1862 mL5.9309 mL

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