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5,6-dihydro-5-Fluorouracil

Name: 5,6-dihydro-5-Fluorouracil
Brand: TargetMol
SKU: T36870
Price: 63 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T36870Cas No. 696-06-0

Alias 5-fluorodihydrouracil, 5-Fluoro-dihydro-pyrimidine-2,4-dione

5,6-dihydro-5-Fluorouracil (5-FUH2) is an inactive metabolite of 5-FU and can be used for the study of organismal metabolism.

5,6-dihydro-5-Fluorouracil

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Catalog No. T36870Alias 5-fluorodihydrouracil, 5-Fluoro-dihydro-pyrimidine-2,4-dioneCas No. 696-06-0

5,6-dihydro-5-Fluorouracil (5-FUH2) is an inactive metabolite of 5-FU and can be used for the study of organismal metabolism.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$63	-	In Stock
5 mg	$169	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	5,6-dihydro-5-Fluorouracil (5-FUH2) is an inactive metabolite of 5-FU and can be used for the study of organismal metabolism.
Targets&IC50	MDA-MB-231 cells:0.25 μg/mL
In vitro	The combination of 5,6-dihydro-5-Fluorouracil (5-Fu) and NSC 123127 (Dox) demonstrates synergistic anticancer efficacy. The IC50 values for 5-Fu/Dox-DNM against human breast cancer (MDA-MB-231) cells are 0.25 μg/mL, with cytotoxicity increasing by 11.2-fold and 6.1-fold for Dox-DNM and 5-Fu-DNM, respectively [4]. In NFBD1-inhibited NPC cells treated with 5,6-dihydro-5-Fluorouracil (5-FU) and CDDP, lentivirus-mediated short hairpin RNA depletion of NFBD1 expression in NPC CNE1 cell line enhances sensitivity. Knockdown of NFBD1 significantly induces apoptosis in CNE1 cells treated with CDDP or 5,6-dihydro-5-Fluorouracil [2].
In vivo	Administered at a dose of 23 mg/kg three times a week for 14 days, 5,6-dihydro-5-Fluorouracil(5-FU) induces gastrointestinal transit acceleration associated with acute intestinal inflammation starting on the third day of treatment. This may lead to sustained changes in the enteric nervous system (ENS) observed beyond the 7th day, and the 14-day treatment results in delayed gastrointestinal transit and colonic motility disorders [1].
Synonyms	5-fluorodihydrouracil, 5-Fluoro-dihydro-pyrimidine-2,4-dione

Chemical Properties

Molecular Weight	132.09
Formula	C₄H₅FN₂O₂
Cas No.	696-06-0
Smiles	FC1CNC(=O)NC1=O

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 43 mg/mL (325.54 mM), Sonication is recommended.

PBS (pH 7.2): 8 mg/mL (60.56 mM), Sonication is recommended.

Ethanol: 0.8 mg/mL (6.06 mM), Sonication is recommended.

DMF: 60 mg/mL (454.24 mM), Sonication is recommended.

Solution Preparation Table

Ethanol/PBS (pH 7.2)/DMSO/DMF

	1mg	5mg	10mg	50mg
1 mM	7.5706 mL	37.8530 mL	75.7060 mL	378.5298 mL
5 mM	1.5141 mL	7.5706 mL	15.1412 mL	75.7060 mL

PBS (pH 7.2)/DMSO/DMF

	1mg	5mg	10mg	50mg
10 mM	0.7571 mL	3.7853 mL	7.5706 mL	37.8530 mL
20 mM	0.3785 mL	1.8926 mL	3.7853 mL	18.9265 mL
50 mM	0.1514 mL	0.7571 mL	1.5141 mL	7.5706 mL

DMSO/DMF

	1mg	5mg	10mg	50mg
100 mM	0.0757 mL	0.3785 mL	0.7571 mL	3.7853 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc