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3'-O-(2-Nitrobenzyl)-dCTP

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Catalog No. TUN2017 Copy Product Info

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3'-O-(2-Nitrobenzyl)-dCTP is a reversible terminator. Recognized and incorporated by polymerases, it temporarily halts primer extension during DNA synthesis. After incorporation, its 3'-O-(2-nitrobenzyl) blocking group can be laser-cleaved to regenerate a free 3'-OH, allowing further primer extension cycles. This compound is useful for resolving homopolymeric regions within DNA templates.

3'-O-
(2-Nitrobenzyl)-dCTP

Cas No. 959755-19-2

Pack Size	Price	USA Stock	Global Stock	Quantity
10 mg	Inquiry	Inquiry	Inquiry
50 mg	Inquiry	Inquiry	Inquiry

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description

In vitro

3'-O-(2-Nitrobenzyl)-dCTP (100 pmol) serves as an effective and quantitative substrate for incorporation by 9°N polymerase (exo-) A485L/Y409V in in vitro DNA extension reactions, resulting in complete primer extension products. At a concentration of 200 pmol, it acts as an efficient reversible terminator for pyrosequencing, allowing accurate single nucleotide resolution analysis of homopolymeric DNA sequences during the extend-signal detection-deprotection cycle.

Chemical Properties

Molecular Weight	602.28
Formula	C₁₆H₂₁N₄O₁₅P₃
Cas No.	959755-19-2
Smiles	O=C1N([C@H]2C[C@H](OCC3=C(N(=O)=O)C=CC=C3)[C@@H](COP(OP(OP(=O)(O)O)(=O)O)(=O)O)O2)C=CC(N)=N1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: 3'-O-(2-Nitrobenzyl)-dCTP in vitro | 3'-O-(2-Nitrobenzyl)-dCTP formula | 3'-O-(2-Nitrobenzyl)-dCTP molecular weight