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3'-O-(2-Nitrobenzyl)-dATP
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Catalog No. TUN2023 Copy Product Info
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3'-O-(2-Nitrobenzyl)-dATP is a reversible, light-sensitive DNA synthesis terminator. It can be incorporated into an extending DNA strand to terminate synthesis in a base-specific manner. Upon UV irradiation, the 3'-protective group of 3'-O-(2-Nitrobenzyl)-dATP can be efficiently removed to restart DNA synthesis, supporting the development of base addition sequencing schemes (BASS) through complete termination-initiation cycles.
3'-O-
(2-Nitrobenzyl)-dATP
Cas No. 159954-97-9
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | 3'-O-(2-Nitrobenzyl)-dATP is a reversible, light-sensitive DNA synthesis terminator. It can be incorporated into an extending DNA strand to terminate synthesis in a base-specific manner. Upon UV irradiation, the 3'-protective group of 3'-O-(2-Nitrobenzyl)-dATP can be efficiently removed to restart DNA synthesis, supporting the development of base addition sequencing schemes (BASS) through complete termination-initiation cycles. |
| In vitro | 3'-O-(2-Nitrobenzyl)-dATP effectively halts DNA synthesis mediated by Bst DNA polymerase at concentrations ranging from 0.25 to 1 mM within 10 minutes. At 250 μM, it can terminate DNA synthesis in a base-specific manner with Bst DNA polymerase, and the 2-nitrobenzyl group can be efficiently removed by 10 minutes of UV irradiation, allowing DNA synthesis to resume. Furthermore, at 250 μM, it induces partial termination of DNA synthesis mediated by AmpliTaq® and VentR® exo- DNA polymerases, causes complete termination with Bst DNA polymerase, and inhibits DNA synthesis mediated by Sequenase®. |
| Molecular Weight | 626.30 |
| Formula | C17H21N6O14P3 |
| Cas No. | 159954-97-9 |
| Smiles | NC1=C2C(N(C=N2)[C@H]3C[C@H](OCC4=C(N(=O)=O)C=CC=C4)[C@@H](COP(OP(OP(=O)(O)O)(=O)O)(=O)O)O3)=NC=N1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: 3'-O-(2-Nitrobenzyl)-dATP in vitro | 3'-O-(2-Nitrobenzyl)-dATP formula | 3'-O-(2-Nitrobenzyl)-dATP molecular weight
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