Powder: -20°C for 3 years | In solvent: -80°C for 1 year
2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection. It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein , but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | 35 days | $ 78.00 | |
5 mg | 35 days | $ 273.00 | |
10 mg | 35 days | $ 458.00 |
Description | 2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection. It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein , but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml). |
Molecular Weight | 886.06 |
Formula | C43H73F6N5O7 |
CAS No. | 1609010-59-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL
Ethanol:PBS (pH 7.2) (1:3): 0.25 mg/mL
Ethanol: 50 mg/mL
DMF: 50 mg/mL
You can also refer to dose conversion for different animals. More
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2H-Cho-Arg (trifluoroacetate salt) 1609010-59-4 2H-Cho-Arg 2H Cho Arg (trifluoroacetate salt) 2HChoArg (trifluoroacetate salt) inhibitor inhibit