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12-HETE

Name: 12-HETE
Brand: TargetMol
SKU: T35507
Rating: 4.5 (1 reviews)

(Synonyms: (±)12-HETE) Copy Product Info

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Synonyms: (±)12-HETE

Catalog No. T35507 Copy Product Info

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12-HETE ((±)12-HETE) is a modulator of PGE2, with both anti-thrombotic and pro-thrombotic effects, inducing PGE2 release and COX-2 expression via phospholipase A2 (sPLA2-IIA) inducers. It is also a neuroregulator activating retinal cell endothelial dysfunction in diabetic retinopathy.

Cas No. 71030-37-0

Pack Size	Price	USA Stock	Global Stock
25 µg	$258	35 days	35 days
50 µg	$490	35 days	35 days
100 µg	$925	35 days	35 days
250 µg	$1,980	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Liquid

Color:Transparent

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Product Introduction

Bioactivity

Description	12-HETE ((±)12-HETE) is a modulator of PGE2, with both anti-thrombotic and pro-thrombotic effects, inducing PGE2 release and COX-2 expression via phospholipase A2 (sPLA2-IIA) inducers. It is also a neuroregulator activating retinal cell endothelial dysfunction in diabetic retinopathy.
In vitro	12-HETE (0.1 μM) activates Muller cells (MCs), enhances glutamate production, and induces inflammation and oxidation. [1] 1 μM exogenous 12-HETE significantly reduced SD-induced cell viability. 12-HETE inhibited SD-induced caspase-3 activity in a concentration-dependent manner with IC50 of 1.13 μM. In OVCAR-3 cells, treatment with 1 μM 12-HETE resulted in increased ILK expression. 1 μM 12-HETE induced the expression of Bcl-2, an anti-apoptotic protein, and inhibited the expression of Bax, a pro-apoptotic protein, and these effects were attenuated by ILK siRNA. [2]
Synonyms	(±)12-HETE

Chemical Properties

Molecular Weight	320.47
Formula	C₂₀H₃₂O₃
Cas No.	71030-37-0
Smiles	C(\C(C/C=C\CCCCC)O)=C/C=C\C/C=C\CCCC(O)=O
Relative Density.	0.984 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: Miscible

DMSO: Miscible

PBS (pH 7.2): 0.8 mg/mL (2.5 mM), Sonication is recommended.

0.1 M Na2CO3: 2 mg/mL (6.24 mM), Sonication is recommended.

DMF: Miscible

Solution Preparation Table

PBS (pH 7.2)/0.1 M Na2CO3

	1mg	5mg	10mg	50mg
1 mM	3.1204 mL	15.6021 mL	31.2042 mL	156.0208 mL

0.1 M Na2CO3

	1mg	5mg	10mg	50mg
5 mM	0.6241 mL	3.1204 mL	6.2408 mL	31.2042 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc