Immobilized Human TSLP (R127A, R130A), His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2 ng/ml determined by ELISA. (QC Test)
Immobilized Human TSLP (R127A, R130A), His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 12.70 nM as determined in BLI assay.
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 0.41 nM as determined in BLI assay.

TSLP Protein, Human, Recombinant (R127A & R130A, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 16.37 kDa and the accession number is Q969D9-1.

Human

HEK293 Cells

TSLP,thymic stromal lymphopoietin

Tyr29-Gln159 (R127A,R130A)

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.