Immobilized Human TNFSF15, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 21.8 ng/ml determined by ELISA.Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15, His Tag with an affinity constant of 12.00 nM as determined in SPR assay (Biacore T200).

TL1A Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.57 kDa and the accession number is A0A0U5JA19.

Human

HEK293 Cells

VEGI192A,VEGI,tumor necrosis factor (ligand) superfamily, member 15,TL1A,TL1

Leu72-Leu251

> 90% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.