MERS-CoV Spike/RBD Protein fragment (aa 367-606, His), Biotinylated

Name: MERS-CoV Spike/RBD Protein fragment (aa 367-606, His), Biotinylated
Brand: TargetMol
SKU: TMPY-05818
Rating: 4.5 (1 reviews)

(Synonyms: RBD Prote, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV) Copy Product Info

Synonyms: RBD Prote, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV

Catalog No. TMPY-05818 Copy Product Info

MERS-CoV Spike/RBD Protein fragment (aa 367-606, His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is K0BRG7.

Pack Size	Price	USA Stock	Global Stock
5 µg	$250	7-10 days	7-10 days
10 µg	$419	7-10 days	7-10 days
20 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	MERS-CoV Spike/RBD Protein fragment (aa 367-606, His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is K0BRG7.
Species	MERS-CoV
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	K0BRG7
Construction	A DNA sequence encoding the receptor binding domain (RBD) of spike protein fragment MERS-CoV (AFS88936.1) (Glu367-Tyr606) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Glu 367
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	RBD Prote, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV
Research Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Chemical Properties

Molecular Weight

27.7 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: MERS-CoV Spike/RBD Protein fragment (aa 367-606, His), Biotinylated chemical structure | MERS-CoV Spike/RBD Protein fragment (aa 367-606, His), Biotinylated molecular weight