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IL-1 beta/IL-1F2 Protein, Human, Recombinant

(Synonyms: interleukin 1, β, interleukin 1, beta, IL1-β, IL1F2, IL1-BETA, IL-1B, IL-1 β, IL-1 beta, IL-1) Copy Product Info

Synonyms: interleukin 1, β, interleukin 1, beta, IL1-β, IL1F2, IL1-BETA, IL-1B, IL-1 β, IL-1 beta, IL-1

Catalog No. TMPY-01049 Copy Product Info
IL-1 beta/IL-1F2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17 kDa and the accession number is P01584.

Batch Information

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Purity:SDS-PAGE: 98.3%; SEC-HPLC: 99.7%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.2-2 ng/mL.
IL-1 beta/IL-1F2 Protein, Human, Recombinant
Description
IL-1 beta/IL-1F2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17 kDa and the accession number is P01584.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberP01584
ConstructionA DNA sequence encoding the mature form of human IL1β (NP_000567.1) (Ala 117-Ser 269) was expressed. Predicted N terminal: Met
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
IL-1 beta/IL-1F2 Protein, Human, RecombinantIL-1 beta/IL-1F2 Protein, Human, Recombinant
Endotoxin< 5 EU/mg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonymsinterleukin 1, β, interleukin 1, beta, IL1-β, IL1F2, IL1-BETA, IL-1B, IL-1 β, IL-1 beta, IL-1
Research Background
Chemical Properties
Molecular Weight17 kDa (predicted); 17 kDa (reducing conditions)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: IL-1 beta/IL-1F2 Protein, Human, Recombinant chemical structure | IL-1 beta/IL-1F2 Protein, Human, Recombinant molecular weight