IL-36RN/IL-1F5 Protein, Human, Recombinant

Name: IL-36RN/IL-1F5 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-00847
Rating: 4.5 (1 reviews)

(Synonyms: PSORS14, PSORP, interleukin 36 receptor antagonist, IL36RN, IL-36Ra, IL36RA, IL1RP3, IL1L1, IL1HY1, IL1F5, FIL1D, FIL1(δ), FIL1(DELTA), FIL1) Copy Product Info

Synonyms: PSORS14, PSORP, interleukin 36 receptor antagonist, IL36RN, IL-36Ra, IL36RA, IL1RP3, IL1L1, IL1HY1, IL1F5, FIL1D, FIL1(δ), FIL1(DELTA), FIL1

Catalog No. TMPY-00847 Copy Product Info

IL-36RN/IL-1F5 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.1 kDa and the accession number is Q9UBH0.

Pack Size	Price	USA Stock	Global Stock
5 µg	$87	7-10 days	7-10 days
10 µg	$142	7-10 days	7-10 days
20 µg	$233	7-10 days	7-10 days
50 µg	$535	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit IL36α-induced IL8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is typically 0.1-0.4 μg/mL in the presence of 20ng/mL IL36α.
Description	IL-36RN/IL-1F5 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 17.1 kDa and the accession number is Q9UBH0.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	Q9UBH0
Construction	A DNA sequence encoding the mature form of human IL1F5 (Q9UBH0) (Met1-Asp155) was expressed. Predicted N terminal: Met
Protein Purity	> 99 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	PSORS14, PSORP, interleukin 36 receptor antagonist, IL36RN, IL-36Ra, IL36RA, IL1RP3, IL1L1, IL1HY1, IL1F5, FIL1D, FIL1(δ), FIL1(DELTA), FIL1
Research Background	Interleukin-1 family member 5 (IL-1F5), also known as interleukin 36 receptor antagonist (IL36RA), is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). IL-1F5 is a highly and specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist, and interleukin-1 receptor type I (IL-1R1), which is present in epithelial barriers and takes part in the local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor, single Ig IL-1R-related molecule (SIGIRR)/TIR8, as the effects were not observed in SIGIRR-/- mice. In contrast to its effects in brain tissue, IL-1F5 did not attenuate LPS-induced changes, or up-regulated IL-4 in macrophages or dendritic cells, suggesting that the effect is confined to the brain.

Chemical Properties

Molecular Weight

17.1 kDa (predicted); 18 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.