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Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc)

(Synonyms: Human cytωlovirus (HCMV) Glycoprotein B/gB) Copy Product Info

Synonyms: Human cytωlovirus (HCMV) Glycoprotein B/gB

Catalog No. TMPY-00812 Copy Product Info
Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 104 kDa and the accession number is P13201.
Pack SizePriceUSA StockGlobal StockQuantity
5 µg$957-10 days7-10 days
10 µg$1557-10 days7-10 days
20 µg$2577-10 days7-10 days
50 µg$4977-10 days7-10 days
100 µg$9877-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Information

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 104 kDa and the accession number is P13201.
Species
CMV
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP13201
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Lys 700) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Asn 25
Protein Purity
> 80 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsHuman cytωlovirus (HCMV) Glycoprotein B/gB
Research Background
Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
Chemical Properties
Molecular Weight104 kDa (predicted); 135-145 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) chemical structure | Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (aa 1-700, hFc) molecular weight