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Siglec-2/CD22 Protein, Mouse, Recombinant (aa 22-702, His)

Catalog No. TMPJ-00444
Synonyms: CD22 antigenMGC130020, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, SIGLEC2FLJ22814, T-cell surface antigen Leu-14, CD22 molecule, Siglec-2, sialic acid binding Ig-like lectin 2, BL-CAM, CD22

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

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Siglec-2/CD22 Protein, Mouse, Recombinant (aa 22-702, His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 71.00
50 μg 5 days $ 215.00
500 μg 5 days $ 1,840.00
1 mg 5 days $ 2,770.00
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Biological Description
Technical Params
Product Properties
Description Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.
Species Mouse
Expression System Human Cells
Tag C-6His
Accession Number AAA02562.1
Synonyms CD22 antigenMGC130020, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, SIGLEC2FLJ22814, T-cell surface antigen Leu-14, CD22 molecule, Siglec-2, sialic acid binding Ig-like lectin 2, BL-CAM, CD22
Amino Acid Ser22-Arg702
Construction Recombinant Mouse B-cell Receptor CD22 is produced by our Mammalian expression system and the target gene encoding Ser22-Arg702 is expressed with a 6His tag at the C-terminus.
Protein Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 100-120 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Stability & Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Research Background Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

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Keywords

Siglec-2/CD22 Protein, Mouse, Recombinant (aa 22-702, His) CD22 antigenMGC130020 SIGLEC2FLJ-22814 CD-22 B-cell receptor CD22 B-lymphocyte cell adhesion molecule CD 22 SIGLEC2FLJ22814 T-cell surface antigen Leu-14 SIGLEC2FLJ 22814 CD22 antigenMGC-130020 CD22 molecule CD22 antigenMGC 130020 Siglec-2 sialic acid binding Ig-like lectin 2 BL-CAM CD22 recombinant recombinant-proteins proteins protein

 

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