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CD117 Protein, Mouse, Recombinant (His)

Catalog No. TMPK-00777
Synonyms: ckit, PBT, SCFR, KIT, CD117, c-kit

The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

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CD117 Protein, Mouse, Recombinant (His)
Pack Size Availability Price/USD Quantity
100 μg 5 days $ 487.00
500 μg 5 days $ 1,950.00
1 mg 5 days $ 3,250.00
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Biological Description
Technical Params
Product Properties
References and Literature
Description The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Species Mouse
Expression System HEK293
Tag C-His
Accession Number P05532-2
Synonyms ckit, PBT, SCFR, KIT, CD117, c-kit
Construction Recombinant Mouse CD117 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser25-Pro523.[Accession |P05532-2]
Protein Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight The protein has a predicted MW of 56.63 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
Endotoxin Less than 1EU per μg by the LAL method.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage

-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

References and Literature

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

CD117 Protein, Mouse, Recombinant (His) ckit CD-117 PBT SCFR KIT CD117 CD 117 c-kit recombinant recombinant-proteins proteins protein

 

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