1. Immobilized Anti-CD20 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus FCGRT&B2M Heterodimer Protein (His & AVI Tag), Biotinylated, the EC50 is 80-240 ng/mL.
2. Loaded Biotinylated Cynomolgus FcRn recombinant protein ( His & Avi Tag ) on SA Biosensor, can bind IgG1 Fc with an affinity constant of 0.08 μM as determined in a BLI assay.
3. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 90% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.

Cynomolgus

HEK293 Cells

Fc fragment of IgG, receptor, transporter, α,Fc fragment of IgG, receptor, transporter, alpha

A DNA sequence encoding the cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.