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Sulfobetaine-12 (Zwittergent 3-12 Detergent) is a zwitterionic surfactant, dispersant and emulsifier commonly used in industry.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 g | $45 | - | In Stock | |
| 10 g | $63 | - | In Stock |
| Description | Sulfobetaine-12 (Zwittergent 3-12 Detergent) is a zwitterionic surfactant, dispersant and emulsifier commonly used in industry. |
| In vitro | In cell culture, Sulfobetaine-12 can be used as a surfactant to help reduce the surface tension of liquids and promote uniform cell distribution and growth. It also reduces cell-to-cell adhesion, allowing for easier cell separation and passaging. In enzymatic studies, Sulfobetaine-12 can help stabilize the structure of enzymes to prevent inactivation during storage or experimental procedures. It can prolong the life of an enzyme by providing a mild environment to maintain the active conformation of the enzyme. [1] |
| Synonyms | Zwittergent 3-12 Detergent |
| Molecular Weight | 335.55 |
| Formula | C17H37NO3S |
| Cas No. | 14933-08-5 |
| Smiles | O=S(=O)([O-])CCC[N+](C)(C)CCCCCCCCCCCC |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 40 mg/mL (119.21 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (5.96 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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