WEE1-IN-7 (compound 12h) is a potent, orally active WEE1 inhibitor with an IC50 value of 2.1 nM. This compound induces apoptosis and causes cell cycle arrest in the S phase, demonstrating antitumor activity.

WEE1-IN-7 (compound 12h) inhibits cell viability with IC 50 values of 84 nM for A427 cells and 82 nM for Lovo cells. At concentrations ranging from 0-800 nM over 48 hours, WEE1-IN-7 induces apoptosis and causes cell cycle arrest in the S phase in a dose-dependent manner.

WEE1-IN-7, administered orally at a dose of 60 mg/kg daily for 27 days, demonstrates antitumor activity in the Lovo CDX xenograft mouse model, achieving a tumor growth inhibition (TGI) rate of 88%.