USP10-IN-3 (compound D1) is a potent inhibitor of USP10, with an IC50 of 7.2 µM. It can suppress cell proliferation, induce apoptosis, and cause cell cycle arrest in the S phase.

USP10-IN-3 (compound D1) inhibits cell proliferation with an IC 50 value of 2.3 µM in Huh-7 cells at concentrations ranging from 0-100 µM. Additionally, when used at 0-10 µM for 24 hours, it induces apoptosis and causes cell cycle arrest in the S phase in a dose-dependent manner. At concentrations of 0-30 µM over 24 hours, USP10-IN-3 also decreases the expression of YAP1, p53, and p21 in a dose-dependent fashion.