Topoisomerase II inhibitor 18

Name: Topoisomerase II inhibitor 18
Brand: TargetMol
SKU: T87552
Rating: 4.5 (1 reviews)

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Catalog No. T87552Cas No. 2382959-65-9

Topoisomerase II inhibitor 18 (Compound IV), a Quinoxaline derivative, exhibits an IC 50 of 7.5 μM in inhibiting topoisomerase II. It impedes PC-3 cell proliferation, arrests the cell cycle at the S phase, and induces apoptosis. Moreover, this compound demonstrates substantial antitumor activity against cancer [1].

Topoisomerase II inhibitor 18

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Catalog No. T87552Cas No. 2382959-65-9

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 mg	Inquiry	10-14 weeks	10-14 weeks
50 mg	Inquiry	10-14 weeks	10-14 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Product Introduction

Bioactivity

Description	Topoisomerase II inhibitor 18 (Compound IV), a Quinoxaline derivative, exhibits an IC 50 of 7.5 μM in inhibiting topoisomerase II. It impedes PC-3 cell proliferation, arrests the cell cycle at the S phase, and induces apoptosis. Moreover, this compound demonstrates substantial antitumor activity against cancer [1].
Targets&IC50	Topoisomerase II:8.49 μM
In vitro	Topoisomerase II inhibitor 18 (0-100 μM) inhibits the proliferation of cancer cells PC-3 and HepG2 with IC50 values of 2.11 μM and 11.03 μM, respectively, and exhibits low cytotoxicity towards normal Vero cells (IC50 = 23.12 μM) [1]. Western Blot analysis [1] conducted on the PC-3 cell line over a 48-hour incubation period at concentrations of 0-100 μM indicates an increase in p53, cleaved caspase-3, and cleaved caspase-8 levels, and a decrease in Bcl-2 levels. Similarly, proliferation assays [1] demonstrate inhibition of cell proliferation in both HepG2 and PC-3 cell lines, under the same concentration and incubation conditions.
In vivo	Topoisomerase II inhibitor 18, administered at 5 mg/kg via intramuscular injection for 21 days, demonstrates antitumor activity and renal protective effects in an albino mice model with Ehrlich solid tumors [1].

Chemical Properties

Molecular Weight	351.47
Formula	C₂₀H₂₁N₃OS
Cas No.	2382959-65-9
Smiles	S(CCC(NCCC)=O)C=1C(=NC2=C(N1)C=CC=C2)C3=CC=CC=C3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc