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Tetrahydrofolic acid (Standard)

Name: Tetrahydrofolic acid (Standard)
Brand: TargetMol
SKU: TMSM-3179
Rating: 4.5 (1 reviews)

(Synonyms: L-Tetrahydrofolic acid, L-5,6,7,8-Tetrahydrofolic acid) Copy Product Info

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Synonyms: L-Tetrahydrofolic acid, L-5,6,7,8-Tetrahydrofolic acid

Catalog No. TMSM-3179 Copy Product Info

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Tetrahydrofolic acid (Standard) is a reference standard for research and analysis in studies involving Tetrahydrofolic acid. Tetrahydrofolic acid (5,6,7,8-tetrahydrofolic acid) is a vitamin B9 derivative that functions as a one-carbon donor in organisms and is involved in growth and metabolism. Tetrahydrofolic acid (Standard) is utilized in biochemical research focused on folate-mediated one-carbon transfer pathways, nucleotide biosynthesis mechanisms, and metabolic flux analysis in cellular proliferation systems.

Cas No. 135-16-0

Pack Size	Price	USA Stock	Global Stock	Quantity
5 mg	$350	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	Tetrahydrofolic acid (Standard) is a reference standard for research and analysis in studies involving Tetrahydrofolic acid. Tetrahydrofolic acid (5,6,7,8-tetrahydrofolic acid) is a vitamin B9 derivative that functions as a one-carbon donor in organisms and is involved in growth and metabolism. Tetrahydrofolic acid (Standard) is utilized in biochemical research focused on folate-mediated one-carbon transfer pathways, nucleotide biosynthesis mechanisms, and metabolic flux analysis in cellular proliferation systems.
In vitro	Methods：Adh5-knockout DT40 cells were treated with gradient concentrations of tetrahydrofolic acid for 3 days and 16 hours respectively. Cytotoxicity, DNA and chromosomal damage were detected, and the aggregation of damage-related proteins was observed to explore the regulatory mechanism of DNA damage response. Results：Tetrahydrofolic acid exerted cytotoxicity on Adh5-deficient and Fanconi anemia-deficient cells, leading to accumulated DNA damage and chromosome breakage . It induced the formation of intracellular FANCD2 and ser139-H2AX foci in a dose-dependent manner [1]. Tetrahydrofolic acid activated the DNA damage response pathway, depleted nucleotide pools and mediated homologous recombination repair [1].
In vivo	Methods：Adh5-knockout mice received daily intraperitoneal injection of tetrahydrofolic acid at 62.5 mg/kg. Hematopoietic function, protein phosphorylation level and survival of hematopoietic progenitor cells were detected. Results：Tetrahydrofolic acid treatment disrupted hematopoietic function, increased the phosphorylation level of ser139-H2AX and reduced the survival rate of hematopoietic progenitor cells, indicating that excessive tetrahydrofolic acid possesses mutagenicity and genotoxicity to bone marrow cells [1].
Synonyms	L-Tetrahydrofolic acid, L-5,6,7,8-Tetrahydrofolic acid

Chemical Properties

Molecular Weight	445.43
Formula	C₁₉H₂₃N₇O₆
Cas No.	135-16-0
Smiles	O=C1C2=C(NCC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)N2)NC(N)=N1
Relative Density.	1.7g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 95 mg/mL (213.28 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2450 mL	11.2251 mL	22.4502 mL	112.2511 mL
5 mM	0.4490 mL	2.2450 mL	4.4900 mL	22.4502 mL
10 mM	0.2245 mL	1.1225 mL	2.2450 mL	11.2251 mL
20 mM	0.1123 mL	0.5613 mL	1.1225 mL	5.6126 mL
50 mM	0.0449 mL	0.2245 mL	0.4490 mL	2.2450 mL
100 mM	0.0225 mL	0.1123 mL	0.2245 mL	1.1225 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: Tetrahydrofolic acid (Standard) chemical structure | Tetrahydrofolic acid (Standard) in vivo | Tetrahydrofolic acid (Standard) in vitro | Tetrahydrofolic acid (Standard) formula | Tetrahydrofolic acid (Standard) molecular weight