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Ribocil-C

Name: Ribocil-C
Brand: TargetMol
SKU: T12722L
Rating: 4.5 (1 reviews)

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Catalog No. T12722LCas No. 1825355-56-3

Alias RibocilC, Ribocil C

Ribocil-C is a selective inhibitor of the bacterial riboflavin riboswitch, acting as a synthetic analogue of flavin mononucleotide (FMN). It suppresses ribB gene expression and inhibits bacterial cell growth.

Ribocil-C

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Purity: 99.69%

Catalog No. T12722LAlias RibocilC, Ribocil CCas No. 1825355-56-3

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Purity:99.69%

Appearance:Solid

Color:White

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COA MS HNMR HPLC

Product Introduction

Bioactivity

Description	Ribocil-C is a selective inhibitor of the bacterial riboflavin riboswitch, acting as a synthetic analogue of flavin mononucleotide (FMN). It suppresses ribB gene expression and inhibits bacterial cell growth.
In vitro	Ribocil-C is a highly selective inhibitor targeting the flavin mononucleotide (FMN) riboswitch and can regulate gene expression in the de novo biosynthesis pathway of riboflavin (RF, vitamin B₂) in Escherichia coli. Additionally, it specifically acts on dual FMN riboswitches, which respectively control RF biosynthesis essential for Staphylococcus aureus growth and pathogenic processes, as well as RF uptake [1]. As a synthetic small-molecule analog of FMN, Ribocil-C binds to the FMN riboswitch in various Gram-negative (GN) bacteria (including Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii), exerting antibacterial effects by suppressing ribB gene expression and RF synthesis [1][2].
In vivo	Ribocil-C (60 mg/kg and 120 mg/kg) showed significant dose-dependent antibacterial effects, lowering the bacterial load (CFU/g) in the spleen by 1.87 and 3.29 log₁₀, respectively, compared to untreated control mice. Additionally, no significant mortality or toxic reactions were observed [2].
Synonyms	RibocilC, Ribocil C

Chemical Properties

Molecular Weight	419.5
Formula	C₂₁H₂₁N₇OS
Cas No.	1825355-56-3
Smiles	O=C1N=C(NC(=C1)C2=CC=CS2)[C@@H]3CN(CC4=CN(C=N4)C=5N=CC=CN5)CCC3
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 85 mg/mL (202.62 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (7.87 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3838 mL	11.9190 mL	23.8379 mL	119.1895 mL
5 mM	0.4768 mL	2.3838 mL	4.7676 mL	23.8379 mL
10 mM	0.2384 mL	1.1919 mL	2.3838 mL	11.9190 mL
20 mM	0.1192 mL	0.5959 mL	1.1919 mL	5.9595 mL
50 mM	0.0477 mL	0.2384 mL	0.4768 mL	2.3838 mL
100 mM	0.0238 mL	0.1192 mL	0.2384 mL	1.1919 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc