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Real Thiol

Name: Real Thiol
Brand: TargetMol
SKU: T12776L
Rating: 4.5 (1 reviews)

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Catalog No. T12776LCas No. 2280796-90-7

Real Thiol, a reversible reaction-based fluorescent probe, can quantitatively monitor the real-time glutathione dynamics in living cells.

Real Thiol

Copy Product Info

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Catalog No. T12776LCas No. 2280796-90-7

Real Thiol, a reversible reaction-based fluorescent probe, can quantitatively monitor the real-time glutathione dynamics in living cells.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	Inquiry	3-6 months	3-6 months
50 mg	Inquiry	3-6 months	3-6 months
100 mg	Inquiry	3-6 months	3-6 months

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description

Real Thiol, a reversible reaction-based fluorescent probe, can quantitatively monitor the real-time glutathione dynamics in living cells.

In vitro

Real Thiol (RT) exhibits ratiometric fluorescence responses over a wide dynamic range when reacting with Glutathione (GSH). RT and its GSH adduct (RT-GSH) show fluorescence maxima at 487 nm and 562 nm with excitation wavelengths at 405 nm and 488 nm, respectively. The dissociation constant (Kd) for the RT-GSH reaction is 3.7 mM. RT's dynamic range (1 to 10 mM) enables monitoring of GSH level changes in both directions in living cells, with 10% of RT reacting with thiolated proteins and 90% with GSH [1].

Chemical Properties

Molecular Weight	411.37
Formula	C₂₀H₁₇N₃O₇
Cas No.	2280796-90-7
Smiles	OC(=O)CN(CC(O)=O)C(=O)C(=C\c1cc2ccc(cc2oc1=O)N1CCC1)\C#N
Relative Density.	1.599 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 101 mg/mL (245.52 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4309 mL	12.1545 mL	24.3090 mL	121.5451 mL
5 mM	0.4862 mL	2.4309 mL	4.8618 mL	24.3090 mL
10 mM	0.2431 mL	1.2155 mL	2.4309 mL	12.1545 mL
20 mM	0.1215 mL	0.6077 mL	1.2155 mL	6.0773 mL
50 mM	0.0486 mL	0.2431 mL	0.4862 mL	2.4309 mL
100 mM	0.0243 mL	0.1215 mL	0.2431 mL	1.2155 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Real Thiol