Procion Blue HB (Reactive Blue 2) is a triazine dye that removes viral particles from plasma protein solutions.

I. Virus Removal
1. Prepare the solution: First, dissolve Reactive Blue 2 in an appropriate solvent, usually water or PBS. The concentration can be adjusted according to the experimental needs, usually 1-5 mg/mL.
2. Mix with plasma protein solution: Add the dissolved Reactive Blue 2 to the plasma protein solution, usually incubate at room temperature for a certain time (such as 1 hour).
3. Virus Removal: After the virus particles bind to Reactive Blue 2, the virus particles and dye can be removed from the plasma by ultrafiltration, centrifugation or other separation techniques.
4. Washing: Finally, wash the plasma protein solution with PBS or other buffer to remove unbound dye and virus particles.
II. Membrane Filtration Technology for Virus Removal
1. Membrane Filtration: Filter the plasma protein solution treated with Reactive Blue 2 through a membrane filter. Usually, a filter membrane with an appropriate pore size is selected to remove virus particles.
2. Monitoring: Use microscopy, flow cytometry or other detection methods to confirm the removal effect of virus particles.
3. Used for virus clearance research
1. Virus infection experiment: Study the virus removal efficiency by mixing the virus sample with plasma protein solution and treating it with Reactive Blue 2.
2. Detection of viral load: Use PCR, ELISA or other virus detection techniques to measure the concentration change of the virus before and after treatment to evaluate the removal effect.