Polyether F127 Diacrylate

Polyether F127 Diacrylate (F127DA) is a triblock copolymer derived from the acrylation of polyethylene glycol–polypropylene glycol–polyethylene glycol. Polyether F127 Diacrylate (F127DA) rapidly crosslinks and cures into a gel under UV and visible light in the presence of photoinitiators. Polyether F127 Diacrylate (F127DA) exhibits thermoresponsive gelation and is used in biomedical materials research, including hydrogel engineering for 3D bioprinting, cell encapsulation systems, and tissue scaffold design, where Polyether F127 Diacrylate (F127DA) supports studies of cell–matrix interactions and biodegradable hydrogel mechanics.

Methods:Polyether F127 Diacrylate hydrogels with concentrations of 5–20% wt/vol were prepared. Human periodontal ligament stem cells (hPDLSCs) were cultured under routine conditions and mechanical loading (0–120 kPa, 1 Hz, 1 hour per day for 7 consecutive days). Cell viability, proliferation and differentiation-related indicators were detected.
Results:
1.Polyether F127 Diacrylate hydrogels exhibited good biocompatibility with hPDLSCs. The cell survival rate was over 90% after 7 days of culture, and all hydrogels supported cell proliferation. Among them, F127DA-5 showed the best proliferation-promoting effect.
2.Under static culture, hydrogels of different concentrations had no significant effects on fibroblastic differentiation of hPDLSCs. Under mechanical loading, F127DA-5 significantly promoted fibroblastic differentiation by activating the Integrin-FAK pathway and inducing cytoskeletal rearrangement, accompanied by increased collagen synthesis and upregulated protein and gene expression of COL-1 and SCX [1].

Methods:Animal experiments were performed with Polyether F127 Diacrylate at different concentrations via subcutaneous implantation, topical application on root surfaces and intra-articular injection.
1.Male C57BL/6 mice received a single subcutaneous implantation of the material at 5–20% wt/vol. A 2-week observation was conducted to evaluate biocompatibility, tissue response, degradation behavior and structural stability.
2.The material was topically applied once onto the root surface in a rat delayed tooth replantation model to explore its effect on periodontal tissue repair.
3.A 3D-printed porous scaffold was fabricated using 15% (w/v) Polyether F127 Diacrylate combined with 3% PEGDA. The scaffold was implanted subcutaneously into nude mice to assess in vivo performance and adipose tissue survival.
4.Polyether F127 Diacrylate at 15% (w/v) was blended with 10% (w/v) GelMA, loaded with 30 μM KGN and 200 ng/mL TGF-β. The mixture was injected into the articular cavity and cross-linked in situ under 405 nm light. Its effect on cartilage regeneration was investigated in a rat osteoarthritis model.
Results:
1.After 2 weeks of subcutaneous implantation in mice, the material presented good biocompatibility without systemic toxicity or local adverse tissue reactions. It underwent slow biodegradation and maintained structural integrity at the implantation site for at least 2 weeks [1].
2.F127DA-5 hydrogel markedly promoted periodontal ligament regeneration and alleviated abnormal healing including root resorption and tooth ankylosis in the rat delayed tooth replantation model [1].
3.The 3D-printed porous scaffold incorporated with PEGDA possessed excellent in vivo biocompatibility and structural stability. It could support adipose tissue survival and is applicable to breast reconstruction [2].
4.The composite system consisting of GelMA, KGN and TGF-β significantly facilitated the regeneration of functional articular cartilage in rats with osteoarthritis [3].