Olanexidine is a broad-spectrum antibacterial agent and antiseptic, effective against both Gram-positive and Gram-negative bacteria. It is commonly utilized in the research of infection and inflammation [1] [2] [3].

Olanexidine demonstrates a broad-spectrum bactericidal effect against both Gram-positive cocci and Gram-negative bacteria at a concentration of 50 μM over 48 hours, showcasing its potent antimicrobial properties [1]. At a dosage of 50 μg/mL during a 24-hour period, it effectively suppresses chronic inflammatory responses in oral mucosal cells, indicating its potential utility in managing oral inflammation [2]. Furthermore, within a concentration range of 0-15000 μg/mL, Olanexidine, specifically in its gluconate form, impairs the attachment capabilities of virus-like particles to the human norovirus binding receptor and promotes the aggregation of these particles, suggesting a novel antiviral application [3]. In a related cell viability assay focusing on various bacterial strains, including Gram-positive cocci, Enterococcus spp., Gram-positive bacilli, and Gram-negative strains, with concentrations up to 1 mg/mL and a brief exposure of 30 seconds, Olanexidine was found to inhibit bacterial activity, achieving MIC values approximately between 6.8 and 1740 μg/mL, thereby confirming its efficacy across a broad concentration range.

Olanexidine, when applied to the skin at concentrations of 1%, 1.5%, and 2% in saline, demonstrates fast-acting and long-lasting bactericidal effects against both transient and resident skin bacterial flora in male cynomolgus monkeys, with optimal efficacy observed at a 1.5% concentration [4].