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Naphthol AS-TR phosphate disodium

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Catalog No. T86963Cas No. 4264-93-1

Naphthol AS-TR phosphate disodium is a water-soluble chromogenic dye widely employed as an enzymatic substrate for detecting alkaline phosphatase activity, as it undergoes enzymatic hydrolysis to yield a measurable colored product, enabling sensitive spectrophotometric monitoring of enzyme activity in biological samples such as serum or urine for diagnostic and biochemical assay applications.

Naphthol AS-TR phosphate disodium

Naphthol AS-TR phosphate disodium

😃Good
Purity: 98.24%
Catalog No. T86963Cas No. 4264-93-1
Naphthol AS-TR phosphate disodium is a water-soluble chromogenic dye widely employed as an enzymatic substrate for detecting alkaline phosphatase activity, as it undergoes enzymatic hydrolysis to yield a measurable colored product, enabling sensitive spectrophotometric monitoring of enzyme activity in biological samples such as serum or urine for diagnostic and biochemical assay applications.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 mg$35-In Stock
50 mg$52-In Stock
100 mg$81-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:98.24%
Appearance:Solid
Color:White
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Product Introduction

Bioactivity
Description
Naphthol AS-TR phosphate disodium is a water-soluble chromogenic dye widely employed as an enzymatic substrate for detecting alkaline phosphatase activity, as it undergoes enzymatic hydrolysis to yield a measurable colored product, enabling sensitive spectrophotometric monitoring of enzyme activity in biological samples such as serum or urine for diagnostic and biochemical assay applications.
Chemical Properties
Molecular Weight435.71
FormulaC18H13ClNNa2O5P
Cas No.4264-93-1
SmilesO=C(C1=CC2=C(C=C1OP([O-])([O-])=O)C=CC=C2)NC3=CC=C(C=C3C)Cl.[Na+].[Na+]
Storage & Solubility Information
Storagekeep away from direct sunlight | -20°C for 2 years, 4°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: 80 mg/mL (183.61 mM), Sonication is recommended.
DMSO: 40 mg/mL (91.8 mM), Sonication is recommended.
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM2.2951 mL11.4755 mL22.9510 mL114.7552 mL
5 mM0.4590 mL2.2951 mL4.5902 mL22.9510 mL
10 mM0.2295 mL1.1476 mL2.2951 mL11.4755 mL
20 mM0.1148 mL0.5738 mL1.1476 mL5.7378 mL
50 mM0.0459 mL0.2295 mL0.4590 mL2.2951 mL
H2O
1mg5mg10mg50mg
100 mM0.0230 mL0.1148 mL0.2295 mL1.1476 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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