MAO-A/B-IN-5 is an orally active inhibitor of MAO-A (IC50 = 73.88 μM) and MAO-B (IC50 = 91.08 μM). It reverses sodium nitroprusside (SNP)-induced brain lipid peroxidation (IC50 = 130.2 μM) and protein carbonylation (IC50 = 229.8 μM). In mice, MAO-A/B-IN-5 exhibits dose-dependent and time-dependent antidepressant-like effects. This compound is applicable in depression research.

MAO-A:73.88 μM

MAO-A/B-IN-5 (Compound 3B) significantly inhibits the activity of MAO-A and MAO-B in mouse brain tissue at concentrations ranging from 25 to 200 μM, with IC50 values of 73.88 μM for MAO-A and 91.08 μM for MAO-B. At concentrations of 100 to 500 μM, MAO-A/B-IN-5 reverses sodium nitroprusside-induced brain lipid peroxidation (IC50 = 130.2 μM) and protein carbonylation (IC50 = 229.8 μM).

MAO-A/B-IN-5 (Compound 3B), at doses of 0.5-5 mg/kg administered via gavage as a single dose, demonstrated antidepressant-like effects in a tail suspension test conducted on male Swiss mice. In a separate study, MAO-A/B-IN-5, given at 50-300 mg/kg via a single gavage dose and observed over 14 days, did not cause mortality or evident toxicity in female Swiss mice, and there were no significant changes in food/water consumption, body weight, or organ oxidative stress.