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LCL521

Name: LCL521
Brand: TargetMol
SKU: T11835
Rating: 4.5 (1 reviews)

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Catalog No. T11835Cas No. 1226851-11-1

LCL521 inhibits lysosomal acid sphingomyelinase (ASMase).LCL521 is an acid ceramidase (ACDase) inhibitor.

LCL521

😃Good

Catalog No. T11835Cas No. 1226851-11-1

LCL521 inhibits lysosomal acid sphingomyelinase (ASMase).LCL521 is an acid ceramidase (ACDase) inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$987	6-8 weeks	6-8 weeks
50 mg	$1,360	6-8 weeks	6-8 weeks
100 mg	$2,190	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Liquid

Color:White

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COA

Product Introduction

Bioactivity

Description	LCL521 inhibits lysosomal acid sphingomyelinase (ASMase).LCL521 is an acid ceramidase (ACDase) inhibitor.
In vitro	LCL521 (1 µM) effectively inhibited ACDase in MCF7 cells, but the effects were transient. LCL521 (10 µM) caused a profound decrease of sphingosine and an increase of ceramide, but additionally affected the processing and regeneration of the ACDase protein, with biphasic and reversible effects on the expression of ACDase, which paralleled the long-term changes of cellular sphingosine and ceramide. The higher concentrations of LCL521 also inhibited Dihydroceramide desaturase (DES-1). In summary, LCL521 exhibits significant effects on ACDase in a dose and time-dependent manner.[2]
In vivo	When PDT-treated SCCVII cells are used to vaccinate SCCVII tumor-bearing mice, adjuvant LCL521 treatment (75 mg/kg; i.p.) resulted in marked retardation of tumor growth. This effect can be attributed to the capacity of LCL521 to effectively restrict the activity of two main immunoregulatory cell populations (Tregs and myeloid-derived suppressor cells, MDSCs) that are known to hinder the efficacy of PDT vaccines. The interaction of LCL521 with PDT-based antitumor mechanisms is dominated by immune system contribution that includes overriding the effects of immunoregulatory cells but could also include a tacit contribution from boosting direct tumor cell kill.[3]

Chemical Properties

Molecular Weight	592.77
Formula	C₃₁H₅₂N₄O₇
Cas No.	1226851-11-1
Smiles	[C@@H]([C@H](NC(CCCCCCCCCCCCC)=O)COC(CN(C)C)=O)(OC(CN(C)C)=O)C1=CC=C(N(=O)=O)C=C1
Relative Density.	1.093 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 148.5 mg/mL (250.52 mM), Sonication is recommended.

Ethanol: 90 mg/mL (151.83 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (5.57 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6870 mL	8.4350 mL	16.8699 mL	84.3497 mL
5 mM	0.3374 mL	1.6870 mL	3.3740 mL	16.8699 mL
10 mM	0.1687 mL	0.8435 mL	1.6870 mL	8.4350 mL
20 mM	0.0843 mL	0.4217 mL	0.8435 mL	4.2175 mL
50 mM	0.0337 mL	0.1687 mL	0.3374 mL	1.6870 mL
100 mM	0.0169 mL	0.0843 mL	0.1687 mL	0.8435 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc