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JH295 hydrate
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Catalog No. T61061
JH295 hydrate is a potent, irreversible, and selective NIMA-related kinase 2 (Nek2) inhibitor that inhibits cellular Nek2 by Cys22 alkylation (IC50 = 770 nM). JH295 hydrate is not active against the mitotic kinases Cdk1, Aurora B, or Plk1, and does not interfere with bipolar spindle assembly or the spindle assembly checkpoint [1].
JH295 hydrate
JH295 hydrate
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Catalog No. T61061
JH295 hydrate is a potent, irreversible, and selective NIMA-related kinase 2 (Nek2) inhibitor that inhibits cellular Nek2 by Cys22 alkylation (IC50 = 770 nM). JH295 hydrate is not active against the mitotic kinases Cdk1, Aurora B, or Plk1, and does not interfere with bipolar spindle assembly or the spindle assembly checkpoint [1].
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 mg | $2,210 | 10-14 weeks | 10-14 weeks |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | JH295 hydrate is a potent, irreversible, and selective NIMA-related kinase 2 (Nek2) inhibitor that inhibits cellular Nek2 by Cys22 alkylation (IC50 = 770 nM). JH295 hydrate is not active against the mitotic kinases Cdk1, Aurora B, or Plk1, and does not interfere with bipolar spindle assembly or the spindle assembly checkpoint [1]. |
| Targets&IC50 | NEK2:770 nM |
| In vitro | Treating RPMI7951 cells with JH295 (Compound 16; concentration range 0.08-20 μM; for 45 minutes) significantly inhibits the activity of wild-type Nek2, achieving an IC50 value of approximately 1.3 μM. Conversely, this treatment exhibits minimal impact on the C22V mutant version of Nek2. Western Blot analysis, employing the same cell line and varying concentrations of JH295 (0.08 μM to 20 μM over a 45-minute incubation period), corroborates these findings, underscoring the compound's potent inhibition of WT Nek2 while demonstrating negligible effects on the C22V mutant. |
| Molecular Weight | 338.36 |
| Formula | C18H18N4O3 |
| Smiles | #N/A |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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