JAK1/2-IN-3

Name: JAK1/2-IN-3
Brand: TargetMol
SKU: T214258
Rating: 4.5 (1 reviews)

Catalog No. T214258 Copy Product Info

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JAK1/2-IN-3 is an inhibitor of both JAK1 and JAK2, demonstrating IC50 values of 14.73 nM and 10.03 nM, respectively. It can induce G2/M phase cell cycle arrest and trigger endogenous apoptosis (apoptosis) by increasing Bax and caspase3/7 levels while reducing Bcl2 expression. JAK1/2-IN-3 is applicable in research related to leukemia, breast cancer, and colon cancer.

JAK1/2-IN-3

Copy Product Info

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Catalog No. T214258

JAK1/2-IN-3

Pack Size	Price	USA Stock	Global Stock	Quantity
10 mg	Inquiry	Inquiry	Inquiry
50 mg	Inquiry	Inquiry	Inquiry

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	JAK1/2-IN-3 is an inhibitor of both JAK1 and JAK2, demonstrating IC50 values of 14.73 nM and 10.03 nM, respectively. It can induce G2/M phase cell cycle arrest and trigger endogenous apoptosis (apoptosis) by increasing Bax and caspase3/7 levels while reducing Bcl2 expression. JAK1/2-IN-3 is applicable in research related to leukemia, breast cancer, and colon cancer.
Targets&IC50	JAK1:14.73 nM
In vitro	JAK1/2-IN-3 (Compound 3i) exhibits IC50 values of 1.02 μM, 1.87 μM, 7.92 μM, and 1.27 μM against HT-29, MCF-7, HL-60, and HCT-116 cells, respectively. It shows low toxicity to normal WI-38 cells (IC50: 19.93 μM). At 1.02 µM for 24 hours, JAK1/2-IN-3 induces apoptosis in HT-29 cells and causes G2/M phase cell cycle arrest. After 48 hours of treatment at 1.02 µM, it increases Bax and caspase 3/7 levels and decreases Bcl2 expression in HT-29 cells.

Chemical Properties

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc