HSD1590 is a highly effective and potent inhibitor of ROCK1 and ROCK2, with IC50 values of 1.22 nM and 0.51 nM, respectively. It demonstrates exceptional binding affinity to ROCK, with Kd values below 2 nM. Moreover, HSD1590 showcases minimal cytotoxicity, making it an ideal compound for various applications.

ROCK1:1.22 nM (IC50), ROCK2:0.51 nM (IC50)

HSD1590, when administered at concentrations ranging from 0.5 to 1 μM for 24 hours, significantly reduces migration, an effect not attributed to cellular mortality but to the inhibition of live cell movement, as further confirmed by studies at concentrations of 0.5-10 μM over 12-24 hours. Cell viability assays using MDA-MB-23 cells at these concentrations for the specified durations demonstrate about 80% viability at 12 hours, declining to 63% after 24 hours, underscoring the compound's effectiveness in inhibiting migration without causing substantial cell death.