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Hcyb1

Catalog No. T61617

Hcyb1 is a potent and specific inhibitor of phosphodiesterase 2 (PDE2). It exhibits a high degree of selectivity for inhibiting PDE2A with an IC50 value of 0.57 μM. In addition, Hcyb1 displays over 250-fold selectivity against other recombinant members of the PDE family. Moreover, its pharmacological actions include neuroprotective and antidepressant-like effects, which are believed to be mediated through the cAMP/cGMP-CREB-BDNF signaling pathway [1].

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Hcyb1, CAS N/A

Pack Size	Availability	Price/USD
25 mg	10-14 weeks	$ 916.00
50 mg	10-14 weeks	$ 1,190.00
100 mg	10-14 weeks	$ 1,860.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Hcyb1 is a potent and specific inhibitor of phosphodiesterase 2 (PDE2). It exhibits a high degree of selectivity for inhibiting PDE2A with an IC50 value of 0.57 μM. In addition, Hcyb1 displays over 250-fold selectivity against other recombinant members of the PDE family. Moreover, its pharmacological actions include neuroprotective and antidepressant-like effects, which are believed to be mediated through the cAMP/cGMP-CREB-BDNF signaling pathway [1].
In vitro	Hcyb1 significantly enhances cGMP levels by 1.7 to 2.3 folds within 10 minutes of exposure at concentrations ranging from 1 to 100 nM, as well as increases both cGMP and cAMP levels after 24 hours at a concentration of 1 nM. Furthermore, Hcyb1 treatment elevates the phosphorylation levels of CREB and BDNF in HT-22 cells over a 24-hour period and promotes HT-22 cell viability along with cGMP and cAMP accumulation. Its effects on cell viability are concentration- and time-dependent, as shown by increased cell viability at 0.1 and 1 nM concentrations after 24 hours, particularly noticeable from 12 to 24 hours at 1 nM, reaching maximal effects at 24 hours. Western blot analysis reveals that at a concentration of 1 nM, Hcyb1 significantly augments the phosphorylation of CREB and upregulates BDNF expression in HT-22 cells after 24 hours.
In vivo	Hcyb1, at doses of 0.5, 1, and 2 mg/kg administered through gavage (i.g.), was found to significantly reduce immobility time in the forced swimming and tail suspension tests, indicating an antidepressant-like activity. This effect was observed without any changes to locomotor function. The study utilized male imprinting control region (ICR) mice weighing between 20 and 25 g as the animal model. The results demonstrate a dose-dependent decrease in immobility time at the administered doses, highlighting Hcyb1's potential therapeutic effect [3].

Molecular Weight	380.44
Formula	C24H20N4O

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Dose Conversion

You can also refer to dose conversion for different animals. More

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Hcyb1 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.