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GFP11 is a protein fusion tag composed of 16 amino acids. It facilitates the presentation of proteins on the outer membrane of Gram-negative bacteria and emits green fluorescence by complementing with externally added GFP1-10. Due to its small molecular weight, GFP11 does not significantly affect protein solubility or expression, nor does it alter the catalytic activity of the enzyme CsBglA. GFP11 is applicable in creating a split GFP system for high-throughput biotechnology and flow cytometry applications.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | GFP11 is a protein fusion tag composed of 16 amino acids. It facilitates the presentation of proteins on the outer membrane of Gram-negative bacteria and emits green fluorescence by complementing with externally added GFP1-10. Due to its small molecular weight, GFP11 does not significantly affect protein solubility or expression, nor does it alter the catalytic activity of the enzyme CsBglA. GFP11 is applicable in creating a split GFP system for high-throughput biotechnology and flow cytometry applications. |
| Molecular Weight | 1826.07 |
| Formula | C79H124N24O24S |
| Cas No. | 862973-56-6 |
| Smiles | [C@H](CC1=CN=CN1)(C(N[C@H](C(N[C@@H](CC2=CC=C(O)C=C2)C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(NCC(N[C@H](C(N[C@@H]([C@@H](C)O)C(O)=O)=O)[C@H](CC)C)=O)=O)C)=O)C)=O)CC(N)=O)=O)[C@@H](C)C)=O)=O)CCC(O)=O)=O)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CC3=CN=CN3)NC([C@@H](NC([C@H](CCCNC(=N)N)N)=O)CC(O)=O)=O)=O)CCSC)=O)[C@H](C)C)=O)CC(C)C)=O |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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