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GDC-0425

Name: GDC-0425
Brand: TargetMol
SKU: T9666
Price: 149 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T9666Cas No. 1200129-48-1

Alias RG-7602

GDC-0425 (RG-7602) is a potent and highly selective inhibitor of Checkpoint kinase 1 (Chk1). This product blocks DNA damage-induced cell cycle arrest by inhibiting Chk1 activity, forcing damaged cells into mitosis and ultimately leading to apoptosis. Research indicates that the activity of GDC-0425 is modulated by the Ras-MEK signaling pathway, and it significantly enhances tumor regression when combined with DNA-damaging agents like Gemcitabine.

GDC-0425

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Catalog No. T9666Alias RG-7602Cas No. 1200129-48-1

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 mg	$149	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	GDC-0425 (RG-7602) is a potent and highly selective inhibitor of Checkpoint kinase 1 (Chk1). This product blocks DNA damage-induced cell cycle arrest by inhibiting Chk1 activity, forcing damaged cells into mitosis and ultimately leading to apoptosis. Research indicates that the activity of GDC-0425 is modulated by the Ras-MEK signaling pathway, and it significantly enhances tumor regression when combined with DNA-damaging agents like Gemcitabine.
In vitro	GDC-0425 dose-dependently inhibits proliferation (0.001-10 mM, 72 h) and induces Chk1 hyperphosphorylation (3 µM, 24 h) in Chk1-positive breast cancer cells. Mechanistic studies demonstrate that GDC-0425-mediated cell death is dependent on Ras-MEK signaling, as blocking this pathway via MEK inhibition or RNAi protects cells from viability loss [3].
In vivo	In NCr nude mice bearing osteosarcoma (143B) or TNBC (HCC1806, HCC70) xenografts, oral GDC-0425 (50-75 mg/kg) partially inhibits tumor growth. Combined treatment with GDC-0425 (administered at 24, 48, and 72 h post-chemo) and Gemcitabine (120 mg/kg, i.p.) leads to significant tumor regression across all models [3].
Synonyms	RG-7602

Chemical Properties

Molecular Weight	321.38
Formula	C₁₈H₁₉N₅O
Cas No.	1200129-48-1
Smiles	N#CC1=NC=C2NC=3N=CC=CC3C2=C1OC4CCN(CC)CC4

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 20 mg/mL (62.23 mM), when pH is adjusted to 3 with HCl. Sonication is recommmended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.1116 mL	15.5579 mL	31.1158 mL	155.5791 mL
5 mM	0.6223 mL	3.1116 mL	6.2232 mL	31.1158 mL
10 mM	0.3112 mL	1.5558 mL	3.1116 mL	15.5579 mL
20 mM	0.1556 mL	0.7779 mL	1.5558 mL	7.7790 mL
50 mM	0.0622 mL	0.3112 mL	0.6223 mL	3.1116 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc