Floramanoside B

Floramanoside B is a flavonol glycoside that exhibits aldose reductase inhibitory activity and, at the same time, scavenges DPPH radicals, demonstrating antioxidant activity.

DPPH free radical scavenging:6.2 μM (SC₅₀), Aldose reductase:13.7 μM

Method: Rat lens homogenate supernatant was used as the crude enzyme source. The reaction system contained 135 mM Na,K-phosphate buffer (pH 7.0), 100 mM Li₂SO₄, 0.03 mM NADPH, and 1 mM DL-glyceraldehyde. Various concentrations of Floramanoside B were added, and the mixture was incubated at 30°C for 30 min. The consumption of NADPH was measured by fluorescence spectroscopy (excitation 360 nm, emission 460 nm) to evaluate the inhibitory activity against aldose reductase (AR).
Result: Floramanoside B exhibited inhibitory activity against aldose reductase, with an IC₅₀ value of 13.7 μM ^[1].
Method: Various concentrations of Floramanoside B were mixed with 100 μM DPPH ethanol solution and 0.1 M acetate buffer (pH 5.5). After 30 min, the change in absorbance was measured at 517 nm, and the DPPH radical scavenging rate was calculated.
Result: Floramanoside B exhibited DPPH radical scavenging activity, with an SC₅₀ value of 6.2 μM ^[1].