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EZ-482

Name: EZ-482
Brand: TargetMol
SKU: T15265
Price: 149 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15265Cas No. 1016456-76-0

Alias EZ482

EZ-482 is an apolipoprotein (apoE) ligand that binds to the C-terminal domain of apoE3 and apoE4 through a unique N-terminal allosteric effect, useful in Alzheimer's disease research.

EZ-482

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Purity: 97.29%

Catalog No. T15265Alias EZ482Cas No. 1016456-76-0

EZ-482 is an apolipoprotein (apoE) ligand that binds to the C-terminal domain of apoE3 and apoE4 through a unique N-terminal allosteric effect, useful in Alzheimer's disease research.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 mg	$149	-	In Stock

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:97.29%

Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	EZ-482 is an apolipoprotein (apoE) ligand that binds to the C-terminal domain of apoE3 and apoE4 through a unique N-terminal allosteric effect, useful in Alzheimer's disease research.
Targets&IC50	ApoE3/ApoE4:~8 μM (Ki)
In vitro	The dissociation constant of EZ-482 from apoE3 and apoE4 is about 8 μM. EZ-482 (10 or 20 μM) does not directly affect apoE binding to lipids. [1]
In vivo	In combination with anti-PD-1, EZ-482 (administered daily by oral force-feeding at a concentration of 15 mg/kg) reversed M2-EXO-induced ICB resistance in a mouse model of MC38 tumors.[2]
Synonyms	EZ482

Chemical Properties

Molecular Weight	498.94
Formula	C₂₃H₁₉ClN₄O₅S
Cas No.	1016456-76-0
Smiles	O=C(NC1=CC(=CC=C1O)S(=O)(=O)NC=2C=CC=C(Cl)C2)CC3=NN(C(=O)C=4C=CC=CC43)C
Relative Density.	1.49 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (120.25 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0042 mL	10.0212 mL	20.0425 mL	100.2125 mL
5 mM	0.4008 mL	2.0042 mL	4.0085 mL	20.0425 mL
10 mM	0.2004 mL	1.0021 mL	2.0042 mL	10.0212 mL
20 mM	0.1002 mL	0.5011 mL	1.0021 mL	5.0106 mL
50 mM	0.0401 mL	0.2004 mL	0.4008 mL	2.0042 mL
100 mM	0.0200 mL	0.1002 mL	0.2004 mL	1.0021 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc