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Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2
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Catalog No. TMAB-00134
Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2 is a Rabbit antibody targeting Apolipoprotein E/APOE3. Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2
Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00134
Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2 is a Rabbit antibody targeting Apolipoprotein E/APOE3. Anti-Apolipoprotein E/APOE3 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apolipoprotein E4) Polyclonal Antibody, Unconjugated (TMAB-00134) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Apolipoprotein E4 (TMAB-00134) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 32 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. Compared with E3 and E4, E2 exhibits the lowest receptor binding affinity. Defects in ApoE are a cause of hyperlipoproteinemia type III due to increased plasma cholesterol and triglycerides levels which are the consequence of impaired clearance of chylomicron and VLDL remnants. |
| Immunogen | KLH conjugated synthetic peptide: human APOE4 |
| Antigen Species | Human |
| Gene ID | 348 |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Lipid transport,Cholesterol Metabolism,Lipoproteins/Apolipoproteins,Endoderm,Cholesterol Metabolism,Lipid metabolism,Heart disease,Neurodegenerative disease,Alzheimer's disease,Neurodegenerative disease,Lipid metabolism,Endoderm |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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