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EV206

Name: EV206
Brand: TargetMol
SKU: T200885
Price: 68 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T200885 Copy Product Info

Purity: 99.92%

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EV206 is a potent Hsp70 inhibitor, demonstrating significant anti-proliferative activity. This compound induces apoptosis and promotes increased protein expression of cleaved PARP and caspase-3, indicating its potential anti-tumor activity.

Cas No. 2247047-81-8

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Pack Size	Price	USA Stock	Global Stock
1 mg	$68	-	In Stock
5 mg	$168	-	In Stock
10 mg	$272	-	In Stock
25 mg	$542	-	In Stock
50 mg	$868	-	In Stock
100 mg	$1,390	-	In Stock
1 mL x 10 mM (in DMSO)	$185	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.92%

Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	EV206 is a potent Hsp70 inhibitor, demonstrating significant anti-proliferative activity. This compound induces apoptosis and promotes increased protein expression of cleaved PARP and caspase-3, indicating its potential anti-tumor activity.
Targets & IC50	SGC-7901 cells:0.27 μM, MGC-803 cells:0.06 μM, LoVo cells:1 μM, SW480 cells:0.38 μM, RKO cells:0.38 μM
In vitro	EV206 (1–10 μM; incubated for 30 minutes at 4°C) binds directly to the full-length Hsp70 protein and its N-terminal domain, but does not bind to the C-terminal domain [1]. In fluorescence-based equilibrium binding assays, EV206 (up to 250 μM; equilibrated at 20°C) exhibits a higher binding affinity for the N-terminal domain of Hsp70 (Kd = 16.60 μM) than for the C-terminal domain (Kd = 42.27 μM)[1]. EV206 (0.05–0.5 μM; 48 h) inhibits the viability of untreated H1299, A549, H460, H226B, and PC9 non-small cell lung cancer (NSCLC) cells; as determined by the crystal violet assay, the IC50 values were all below 0.5 μM after 48 h of treatment [1]. EV206 (0.05–0.5 μM; 2–3 weeks of culture) dose-dependently inhibited the adhesion-dependent colony formation of H1299, A549, H460, H226B, and PC9 non-small cell lung cancer (NSCLC) cells [1]. EV206 (0.1–0.5 μM; 48 h) induces apoptotic cell death in a dose-dependent manner in H1299, A549, H460, H226B, and PC9 non-small cell lung cancer (NSCLC) cells [1]. EV206 (0.1–0.5 μM; 48 h) dose-dependently reduces Hsp70 and Akt protein levels in H1299, A549, and H460 non-small cell lung cancer (NSCLC) cells, but does not affect Hsp90 protein levels [1]. EV206 (0.1–0.25 μM; 48 h) reduces the stability of Hsp70 protein in H460 non-small cell lung cancer cells via the ubiquitin-proteasome system, as confirmed by the reversal of Hsp70 downregulation following co-treatment with the proteasome inhibitor MG132 (treated at 10 μM for 6 h)[1]. EV206 (0.05–0.25 μM; 2–3 weeks of culture) dose-dependently inhibits the spheroid-forming ability of H1299, A549, and H460 non-small cell lung cancer (NSCLC) cells [1]. EV206 (0.05–0.25 μM; 48 h) reduces the activity of aldehyde dehydrogenase (ALDH) in H460 non-small cell lung cancer (NSCLC) cells; ALDH is a marker of the cancer stem cell (CSC)-like phenotype [1]. EV206 (0.1 μM during spheroid culture) significantly downregulates the mRNA expression of the cancer stem cell-associated markers POU5F1, NANOG, and SOX2 in H460 non-small cell lung cancer spheroids [1].
In vivo	EV206 (10 mg/kg, administered intraperitoneally once every other day for 16 days) significantly inhibited the growth of H460 NSCLC xenografts in Balb/c nude mice without causing a detectable decrease in body weight [1].

Chemical Properties

Molecular Weight	329.40
Formula	C₂₁H₁₉N₃O
Cas No.	2247047-81-8
Smiles	O=C1C=2C=CC=CC2N(CC=C)C3C=4NC=5C=CC=CC5C4CCN13

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (121.43 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0358 mL	15.1791 mL	30.3582 mL	151.7911 mL
5 mM	0.6072 mL	3.0358 mL	6.0716 mL	30.3582 mL
10 mM	0.3036 mL	1.5179 mL	3.0358 mL	15.1791 mL
20 mM	0.1518 mL	0.7590 mL	1.5179 mL	7.5896 mL
50 mM	0.0607 mL	0.3036 mL	0.6072 mL	3.0358 mL
100 mM	0.0304 mL	0.1518 mL	0.3036 mL	1.5179 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: EV206 chemical structure | EV206 in vivo | EV206 in vitro | EV206 formula | EV206 molecular weight