Shopping Cart

Remove All

Your shopping cart is currently empty

Proceed to checkout Continue shopping

EC 144

Name: EC 144
Brand: TargetMol
SKU: T22758
Rating: 4.5 (1 reviews)

😃Good

Catalog No. T22758Cas No. 911397-80-3

Alias EC144

EC 144, a second-generation selective inhibitor of Hsp90 (heat shock protein 90), inhibits tumor growth with an IC50= 1.1 nM for Hsp90α and an EC50 = 14 nM for degradation of Her-2 in MCF-7 cells.

EC 144

😃Good

Catalog No. T22758Alias EC144Cas No. 911397-80-3

EC 144, a second-generation selective inhibitor of Hsp90 (heat shock protein 90), inhibits tumor growth with an IC50= 1.1 nM for Hsp90α and an EC50 = 14 nM for degradation of Her-2 in MCF-7 cells.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
50 mg	$1,980	6-8 weeks	6-8 weeks
100 mg	$2,500	6-8 weeks	6-8 weeks

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Resource Download

COA

Product Introduction

Bioactivity

Description	EC 144, a second-generation selective inhibitor of Hsp90 (heat shock protein 90), inhibits tumor growth with an IC50= 1.1 nM for Hsp90α and an EC50 = 14 nM for degradation of Her-2 in MCF-7 cells.
In vitro	EC 144 is a synthetic Hsp90 inhibitor, blocks LPS-induced TLR4 signaling in RAW 264.7 cells by inhibiting activation of ERK1/2, MEK1/2, JNK, and p38 MAPK but not NF-κB. EC 144 inhibited CD4(+) T cell proliferation in both mouse and human mixed lymphocyte reactions (MLRs), indicating an immunosuppressive effect on adaptive immune activation. [1]
In vivo	In mice treated with EC 144, peritoneal CD11b(+) exudate cells showed reduced phosphorylation of tumor progression locus 2, MEK1/2, and ERK1/2 upon LPS challenge. This corresponded with resistance to LPS-induced systemic inflammation and suppressed TNF-α release. In a mouse collagen-induced arthritis model, EC 144 suppressed arthritis development, correlated with a reduction in antigen-specific antibody production and impaired activation of antigen-specific CD4(+) T cells. [1]
Synonyms	EC144

Chemical Properties

Molecular Weight	413.9
Formula	C₂₁H₂₄ClN₅O₂
Cas No.	911397-80-3
Smiles	ClC1=NC(=NC2=C1C(C#CCC(O)(C)C)=CN2CC3=NC=C(C(OC)=C3C)C)N
Relative Density.	1.29±0.1 g/cm3(20 °C , 760mmHg)

Storage & Solubility Information

Storage

keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (120.8 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.83 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4160 mL	12.0802 mL	24.1604 mL	120.8021 mL
5 mM	0.4832 mL	2.4160 mL	4.8321 mL	24.1604 mL
10 mM	0.2416 mL	1.2080 mL	2.4160 mL	12.0802 mL
20 mM	0.1208 mL	0.6040 mL	1.2080 mL	6.0401 mL
50 mM	0.0483 mL	0.2416 mL	0.4832 mL	2.4160 mL
100 mM	0.0242 mL	0.1208 mL	0.2416 mL	1.2080 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc