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DTT

Name: DTT
Brand: TargetMol
SKU: T5370
Price: 45 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5370Cas No. 3483-12-3

Alias DL-Dithiothreitol

DTT (DL-Dithiothreitol) is a strong reducing agent. DTT has anti-disulfide ptosis activity and, upon oxidation, forms a stable six-membered ring with internal disulfide bonds.

DTT

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Purity: 99.91%

Catalog No. T5370Alias DL-DithiothreitolCas No. 3483-12-3

DTT (DL-Dithiothreitol) is a strong reducing agent. DTT has anti-disulfide ptosis activity and, upon oxidation, forms a stable six-membered ring with internal disulfide bonds.

Pack Size	Price	Availability	Quantity
500 mg	$45	In Stock

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Purity:99.91%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	DTT (DL-Dithiothreitol) is a strong reducing agent. DTT has anti-disulfide ptosis activity and, upon oxidation, forms a stable six-membered ring with internal disulfide bonds.
Targets&IC50	5-LOX:0.42 µM ± 0.11 log units, NMN:250 μM/mg
In vitro	METHODS: DLD-1 and HCT116 cells under glucose starvation were treated with DTT (0.5 mM) for 12 hours, and PI-positive dead cells were analyzed by flow cytometry. RESULTS: DTT can prevent disulfide stress in DLD-1 and HCT116 cells under glucose starvation and inhibit disulfide death of the cells. [1]
Synonyms	DL-Dithiothreitol

Chemical Properties

Molecular Weight	154.25
Formula	C₄H₁₀O₂S₂
Cas No.	3483-12-3
Smiles	[C@H]([C@@H](CS)O)(CS)O
Relative Density.	1.302g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store under nitrogen | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 200 mg/mL (1296.6 mM), Sonication is recommended.

DMSO: 242 mg/mL (1568.88 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (32.41 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	6.4830 mL	32.4149 mL	64.8298 mL	324.1491 mL
5 mM	1.2966 mL	6.4830 mL	12.9660 mL	64.8298 mL
10 mM	0.6483 mL	3.2415 mL	6.4830 mL	32.4149 mL
20 mM	0.3241 mL	1.6207 mL	3.2415 mL	16.2075 mL
50 mM	0.1297 mL	0.6483 mL	1.2966 mL	6.4830 mL
100 mM	0.0648 mL	0.3241 mL	0.6483 mL	3.2415 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc